Glucagon-like peptide-2 increases sucrase-isomaltase but not caudal-related homeobox protein-2 gene expression

P A Kitchen, A J Fitzgerald, R A Goodlad, N F Barley, M A Ghatei, S Legon, S R Bloom, A Price, J R Walters, A Forbes

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34 Citations (Scopus)

Abstract

To determine the effect of glucagon-like peptide-2 (GLP-2) on sucrase-isomaltase and caudal-related homeobox protein-2 (Cdx-2) gene expression, male Wistar rats were divided into total parenteral nutrition (TPN)-fed and GLP-2-treated, TPN-fed groups. TPN was given via a jugular line, inserted under anesthesia, for 7 days. The treatment group received 40 microg/day of GLP-2 intravenously with the TPN diet. The small intestine and colon were weighed and measured. Tissue was obtained from the jejunum, terminal ileum, and midcolon. RNA analysis, morphometry, and microdissection were performed. The weight of the small intestine of GLP-2-treated rats was greater than that of TPN-fed rats (P
Original languageEnglish
Pages (from-to)G425-8
JournalAJP: Gastrointestinal and Liver Physiology
Volume278
Issue number3
DOIs
Publication statusPublished - Mar 2000

Keywords

  • Animals
  • Colon
  • Gene Expression
  • Glucagon-Like Peptide 2
  • Glucagon-Like Peptides
  • Homeodomain Proteins
  • Ileum
  • Jejunum
  • Male
  • Parenteral Nutrition, Total
  • Peptides
  • Rats
  • Rats, Wistar
  • Sucrase-Isomaltase Complex
  • Trans-Activators

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