Halocarbon emissions by selected tropical seaweeds exposed to different temperatures

Fiona Seh Lin Keng, Siew-Moi Phang, Noorsaadah Abd Rahman, Hui-Yin Yeong , Gill Malin, Emma Elvidge, Bill Sturges

Research output: Contribution to journalArticlepeer-review

Abstract

Four tropical seaweeds, Gracilaria manilaensis Yamamoto & Trono, Ulva reticulata Forsskål, Kappaphycus alvarezii (Doty) L.M.Liao and Turbinaria conoides (J.Agardh) Kützing, collected from various habitats throughout Malaysia, were subjected to temperatures of 40, 35, 30, 25 and 20 ◦C in the laboratory. An exposure range of 21–38 ◦C is reported for Malaysian waters. The effect of the temperature exposures on the halocarbon emissions of the seaweeds were determined 4 and 28 h after treatment. The emission rates for a suite of six halocarbons commonly emitted by seaweeds, bromoform (CHBr3), dibromomethane (CH2Br2), diiodomethane (CH2I2), iodomethane (CH3I), dibromochloromethane (CHBr2Cl) and dichlorobromomethane (CHBrCl2), were measured using a cryogenic purge-and-trap sample preparation system coupled to a gas chromatography–mass spectrometry. The emission rate of CHBr3 was the highest of the six halocarbons for all the seaweeds under all the temperatures tested, followed by CH2Br2, and CH2I2. The emission rates were affected by temperature change and exposure duration, but overall responses were unique to each seaweed species. Larger decreases in the emissions of CHBr3, CH2Br2, CH2I2 and CHBr2Cl were found for K. alvarezii and T. conoides after 4 h at 40 ◦C. In both cases there was a >90% (p < 0.05) reduction in the Fv/Fm value suggesting that photosynthetic actitivity was severely compromised. After a 28 h exposure period, strong negative correlations (r = -0.69 to -0.95; p < 0.01) were observed between temperature and the emission of CHBr3, CH2Br2 and CH2I2 for U. reticulata, K. alvarezii and T. conoides. This suggests a potential decrease in the halocarbon emissions from these tropical seaweeds, especially where the temperature increase is a prolonged event. Strong correlations were also seen between seaweed chlorophyll and carotenoid pigment contents and the emission rates for CHBr3, CH2Br2 and CH2I2 (r = 0.48 to 0.96 and -0.49 to -0.96; p < 0.05). These results suggest that the regulation of halocarbon production versus reactive oxygen species production in seaweeds is an area worthy of further exploration.
Original languageEnglish
Article number112869
JournalPhytochemistry
Volume190
Early online date15 Jul 2021
DOIs
Publication statusPublished - Oct 2021

Keywords

  • Bromoform
  • Climate change
  • Gracilaria manilaensis (Gracilariaceae)
  • Halocarbon emissions
  • Kappaphycus alvarezii (Solieriaceae)
  • Temperature
  • Tropical seaweeds
  • Turbinaria conoides (Sargassaceae)
  • Ulva reticulata (Ulvaceae)

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