TY - JOUR
T1 - Harnessing precursor-directed biosynthesis with glucose derivatives to access cotton fibers with enhanced physical properties
AU - Kuperman, Ofir Aharon
AU - de Andrade, Peterson
AU - Sui, Xiao Meng
AU - Maria, Raquel
AU - Kaplan-Ashiri, Ifat
AU - Jiang, Qixiang
AU - Terlier, Tanguy
AU - Kirkensgaard, Jacob Judas Kain
AU - Field, Robert A.
AU - Natalio, Filipe
N1 - Acknowledgements: We want to thank Dr. Samuel Bod\u00E9 and Dr. Katja Van Nieuland (Isotope Bioscience Laboratory (ISOFYS), Faculty of Bioscience Engineering, Belgium) for their help with EA-IRMS measurements. We want to thank Luis Favas for the illustrations. This work was funded by the European Union, ERC Consolidator project BIOMATFAB (project #101045466); a GIF German-Israeli Foundation for Scientific Research and Development research grant #I-1509-302.5/2019; the MINERVA Stiftung (project # 136809), with the funds from the BMBF of the Federal Republic of Germany, Benoziyo Endowment Fund for the Advancement of Science; a research grant from the Yotam project, the Weizmann Institute Sustainability and Energy Research Initiative (SAERI); the Moskowitz Center at the Weizmann Institute of Science for Nano and Bio-Imaging funding; and the University of Manchester. Views and opinions expressed are, however, those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council. Neither the European Union nor the granting authority can be held responsible for them. ToF-SIMS analysis was carried out with support provided by the National Science Foundation CBET-1626418. This work was conducted in part using resources of the Shared Equipment Authority at Rice University (USA). Data were generated in part by accessing research infrastructure at University of Copenhagen, including FOODHAY (Food and Health Open Innovation Laboratory, Danish Roadmap for Research Infrastructure). O.A.K. performed growth cotton ovules in vitro experiments, performed single-fiber mechanical testing, and wrote and revised the manuscript. P.A. performed the chemosynthesis of 6F-Glc-1P and wrote and revised the manuscript. X.S. performed the wettability experiments, analyzed and interpreted the data, and wrote and revised the manuscript. R.M. performed the cross-sections and the elemental analysis of the fibers' cross-sections, analyzed and interpreted the data, and wrote and revised the manuscript. I.K.-A. performed the wettability experiments, analyzed and interpreted the data, and wrote and revised the manuscript. Q.J. performed the water sorption dynamics measurements, analyzed and interpreted the data, and wrote and revised the manuscript. T.T. performed the ToF-SIMS analysis of the 6F-Glc-1P and control fibers, analyzed and interpreted the data, and wrote and revised the manuscript. J.J.K.K. performed WAXS analysis 6F-Glc-1P and control fibers, analyzed and interpreted the data, and wrote and revised the manuscript. R.F. performed conceptualization, supervision, management, and funding acquisition and wrote and revised the manuscript. F.N. performed conceptualization, supervision, management, and funding acquisition and wrote and revised the manuscript. The authors declare no competing interests.
PY - 2024/5/15
Y1 - 2024/5/15
N2 - Cotton ovule in vitro cultures are a promising platform for exploring biofabrication of fibers with tailored properties. When the ovules' growth medium is supplemented with chemically synthesized cellulose precursors, it results in their integration into the developing fibers, thereby tailoring their end properties. Here, we report the feeding of synthetic glucosyl phosphate derivative, 6-deoxy-6-fluoro-glucose-1-phosphate (6F-Glc-1P) to cotton ovules growing in vitro, demonstrating the metabolic incorporation of 6F-Glc into the fibers with enhanced mechanical properties and moisture-retention capacity while emphasizing the role of molecular hierarchical architecture in defining functional characteristics and mechanical properties. This incorporation strategy bypasses the early steps of conventional metabolic pathways while broadening the range of functionalities that can be employed to customize fiber end properties. Our approach combines materials science, chemistry, and plant sciences to illustrate the innovation required to find alternative solutions for sustainable production of functional cotton fibers with enhanced and emergent properties.
AB - Cotton ovule in vitro cultures are a promising platform for exploring biofabrication of fibers with tailored properties. When the ovules' growth medium is supplemented with chemically synthesized cellulose precursors, it results in their integration into the developing fibers, thereby tailoring their end properties. Here, we report the feeding of synthetic glucosyl phosphate derivative, 6-deoxy-6-fluoro-glucose-1-phosphate (6F-Glc-1P) to cotton ovules growing in vitro, demonstrating the metabolic incorporation of 6F-Glc into the fibers with enhanced mechanical properties and moisture-retention capacity while emphasizing the role of molecular hierarchical architecture in defining functional characteristics and mechanical properties. This incorporation strategy bypasses the early steps of conventional metabolic pathways while broadening the range of functionalities that can be employed to customize fiber end properties. Our approach combines materials science, chemistry, and plant sciences to illustrate the innovation required to find alternative solutions for sustainable production of functional cotton fibers with enhanced and emergent properties.
KW - cotton fibers
KW - metabolic incorporation
KW - modified properties
KW - synthetic glucose derivatives
UR - http://www.scopus.com/inward/record.url?scp=85192964299&partnerID=8YFLogxK
U2 - 10.1016/j.xcrp.2024.101963
DO - 10.1016/j.xcrp.2024.101963
M3 - Article
AN - SCOPUS:85192964299
VL - 5
JO - Cell Reports Physical Science
JF - Cell Reports Physical Science
SN - 2666-3864
IS - 5
M1 - 101963
ER -