High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes

Ángela Ruiz-Tórtola, Francisco Prats-Quílez, Daniel González-Lucas, María-José Bañuls, Ángel Maquieira, Guy Wheeler, Tamas Dalmay, Amadeu Griol, Juan Hurtado, Jaime García-Rupérez

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Abstract

A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods.
Original languageEnglish
Pages (from-to)1717-1727
Number of pages11
JournalBiomedical Optics Express
Volume9
Issue number4
DOIs
Publication statusPublished - 14 Mar 2018

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