High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes

Ángela Ruiz-Tórtola; Francisco Prats-Quílez; Daniel González-Lucas; María-José Bañuls; Ángel Maquieira; Guy Wheeler; Tamas Dalmay; Amadeu Griol; Juan Hurtado; Jaime García-Rupérez

doi:10.1364/BOE.9.001717

High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes

Ángela Ruiz-Tórtola, Francisco Prats-Quílez, Daniel González-Lucas, María-José Bañuls, Ángel Maquieira, Guy Wheeler, Tamas Dalmay, Amadeu Griol, Juan Hurtado, Jaime García-Rupérez

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

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Abstract

A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods.

Original language	English
Pages (from-to)	1717-1727
Number of pages	11
Journal	Biomedical Optics Express
Volume	9
Issue number	4
DOIs	https://doi.org/10.1364/BOE.9.001717
Publication status	Published - 14 Mar 2018

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Tamas Dalmay
- T.Dalmayuea.acuk
- School of Biological Sciences - Professor of RNA Biology
- Plant Sciences - Member
Person: Research Group Member, Academic, Teaching & Research

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Ruiz-Tórtola, Á., Prats-Quílez, F., González-Lucas, D., Bañuls, M-J., Maquieira, Á., Wheeler, G., Dalmay, T., Griol, A., Hurtado, J., & García-Rupérez, J. (2018). High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes. Biomedical Optics Express, 9(4), 1717-1727. https://doi.org/10.1364/BOE.9.001717

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title = "High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes",

abstract = "A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods.",

author = "{\'A}ngela Ruiz-T{\'o}rtola and Francisco Prats-Qu{\'i}lez and Daniel Gonz{\'a}lez-Lucas and Mar{\'i}a-Jos{\'e} Ba{\~n}uls and {\'A}ngel Maquieira and Guy Wheeler and Tamas Dalmay and Amadeu Griol and Juan Hurtado and Jaime Garc{\'i}a-Rup{\'e}rez",

year = "2018",

month = mar,

day = "14",

doi = "10.1364/BOE.9.001717",

language = "English",

volume = "9",

pages = "1717--1727",

journal = "Biomedical Optics Express",

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Ruiz-Tórtola, Á, Prats-Quílez, F, González-Lucas, D, Bañuls, M-J, Maquieira, Á, Wheeler, G, Dalmay, T, Griol, A, Hurtado, J & García-Rupérez, J 2018, 'High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes', Biomedical Optics Express, vol. 9, no. 4, pp. 1717-1727. https://doi.org/10.1364/BOE.9.001717

High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes. / Ruiz-Tórtola, Ángela; Prats-Quílez, Francisco; González-Lucas, Daniel et al.

In: Biomedical Optics Express, Vol. 9, No. 4, 14.03.2018, p. 1717-1727.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes

AU - Ruiz-Tórtola, Ángela

AU - Prats-Quílez, Francisco

AU - González-Lucas, Daniel

AU - Bañuls, María-José

AU - Maquieira, Ángel

AU - Wheeler, Guy

AU - Dalmay, Tamas

AU - Griol, Amadeu

AU - Hurtado, Juan

AU - García-Rupérez, Jaime

PY - 2018/3/14

Y1 - 2018/3/14

N2 - A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods.

AB - A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods.

U2 - 10.1364/BOE.9.001717

DO - 10.1364/BOE.9.001717

M3 - Article

VL - 9

SP - 1717

EP - 1727

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 4

ER -

High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes

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Tamas Dalmay

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