High Spatial Resolution Luciferase Imaging of the Arabidopsis thaliana Circadian Clock

Mark Greenwood, Anthony J.W. Hall, James C.W. Locke

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

The A. thaliana circadian clock is an example of a gene network that generates rich temporal and spatial dynamics. Bioluminescent imaging has proven a powerful method to help dissect the genetic mechanisms that generate oscillations of gene expression over the course of the day. However, its use for the study of spatial regulation is often limited by resolution. Here, we describe a modified luciferase imaging method for the study of the Arabidopsis circadian clock across the plant at sub-tissue-level resolution.

Original languageEnglish
Title of host publicationPlant Circadian Networks
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc
Pages47-55
Number of pages9
Edition2
ISBN (Electronic)978-1-0716-1912-4
ISBN (Print)978-1-0716-1911-7, 978-1-0716-1914-8
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2398
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Circadian clock
  • Coordination
  • High resolution
  • Imaging
  • Luciferase
  • Spatial
  • Tissue specific

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