High-stringency subtraction for the identification of differentially regulated cDNA clones

C.P. Scutt, P.M. Gilmartin

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


The technique of high-stringency subtraction described here facilitates subtractive hybridizations between directional cDNA libraries constructed in ? ZAP® II cloning vectors and represents an improvement on earlier methods for the subtraction of entire cDNA libraries. High-stringency subtraction is designed to eliminate the subtraction of differentially expressed cDNAs, which show similarity to constitutive sequences by the incorporation of a novel high-stringency wash step. This method also allows the size-selection of target cDNAs and incorporates an improved procedure for the synthesis of driver DNA used in subtractions.
Original languageEnglish
Pages (from-to)468-474
Number of pages7
Issue number3
Publication statusPublished - 1 Sep 1997

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