Abstract
The technique of high-stringency subtraction described here facilitates subtractive hybridizations between directional cDNA libraries constructed in ? ZAP® II cloning vectors and represents an improvement on earlier methods for the subtraction of entire cDNA libraries. High-stringency subtraction is designed to eliminate the subtraction of differentially expressed cDNAs, which show similarity to constitutive sequences by the incorporation of a novel high-stringency wash step. This method also allows the size-selection of target cDNAs and incorporates an improved procedure for the synthesis of driver DNA used in subtractions.
| Original language | English |
|---|---|
| Pages (from-to) | 468-474 |
| Number of pages | 7 |
| Journal | Biotechniques |
| Volume | 23 |
| Issue number | 3 |
| Publication status | Published - 1 Sep 1997 |