Abstract
A new satellite RNA (satRNA) of grapevine fanleaf virus (GFLV) was identified by high-throughput sequencing of high-definition (HD) adapter libraries from grapevine plants of the cultivar Panse precoce (PPE) affected by enation disease. The complete nucleotide sequence was obtained by automatic sequencing using primers designed based on next-generation sequencing (NGS) data. The full-length sequence, named satGFLV-PPE, consisted of 1119 nucleotides with a single open reading frame from position 15 to 1034. This satRNA showed maximum nucleotide sequence identity of 87 % to satArMV-86 and satGFLV-R6. Symptomatic grapevines were surveyed for the presence of the satRNA, and no correlation was found between detection of the satRNA and enation symptom expression.
Original language | English |
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Pages (from-to) | 1401-1403 |
Number of pages | 3 |
Journal | Archives of Virology |
Volume | 161 |
Issue number | 5 |
Early online date | 12 Feb 2016 |
DOIs | |
Publication status | Published - May 2016 |
Profiles
-
Tamas Dalmay
- School of Biological Sciences - Professor of RNA Biology
- Plant Sciences - Member
Person: Research Group Member, Academic, Teaching & Research