High-throughput-sequencing-based identification of a grapevine fanleaf virus satellite RNA in Vitis vinifera

Michela Chiumenti, Irina Mohorianu, Vincenzo Roseti, Pasquale Saldarelli, Tamas Dalmay, Angelantonio Minafra

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A new satellite RNA (satRNA) of grapevine fanleaf virus (GFLV) was identified by high-throughput sequencing of high-definition (HD) adapter libraries from grapevine plants of the cultivar Panse precoce (PPE) affected by enation disease. The complete nucleotide sequence was obtained by automatic sequencing using primers designed based on next-generation sequencing (NGS) data. The full-length sequence, named satGFLV-PPE, consisted of 1119 nucleotides with a single open reading frame from position 15 to 1034. This satRNA showed maximum nucleotide sequence identity of 87 % to satArMV-86 and satGFLV-R6. Symptomatic grapevines were surveyed for the presence of the satRNA, and no correlation was found between detection of the satRNA and enation symptom expression.

Original languageEnglish
Pages (from-to)1401-1403
Number of pages3
JournalArchives of Virology
Volume161
Issue number5
Early online date12 Feb 2016
DOIs
Publication statusPublished - May 2016

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