Host association of Campylobacter genotypes transcends geographic variation

Samuel K. Sheppard, Frances Colles, Judith Richardson, Alison J. Cody, Richard Elson, Andrew Lawson, Geraldine Brick, Richard Meldrum, Christine L. Little, Robert J. Owen, Martin C. J. Maiden, Noel D. McCarthy

Research output: Contribution to journalArticlepeer-review

Abstract

Genetic attribution of bacterial genotypes has become a major tool in the investigation of the epidemiology of campylobacteriosis and has implicated retail chicken meat as the major source of human infection in several countries. To investigate the robustness of this approach to the provenance of the reference data sets used, a collection of 742 Campylobacter jejuni and 261 Campylobacter coli isolates obtained from United Kingdom-sourced chicken meat was established and typed by multilocus sequence typing. Comparative analyses of the data with those from other isolates sourced from a variety of host animals and countries were undertaken by genetic attribution, genealogical, and population genetic approaches. The genotypes from the United Kingdom data set were highly diverse, yet structured into sequence types, clonal complexes, and genealogical groups very similar to those seen in chicken isolates from the Netherlands, the United States, and Senegal, but more distinct from isolates obtained from ruminant, swine, and wild bird sources. Assignment analyses consistently grouped isolates from different host animal sources regardless of geographical source; these associations were more robust than geographic associations across isolates from three continents. We conclude that, notwithstanding the high diversity of these pathogens, there is a strong signal of association of multilocus genotypes with particular hosts, which is greater than the geographic signal. These findings are consistent with local and international transmission of host-associated lineages among food animal species and provide a foundation for further improvements in genetic attribution.
Original languageEnglish
Pages (from-to)5269-5277
Number of pages9
JournalApplied and Environmental Microbiology
Volume76
Issue number15
Early online date4 Jun 2010
DOIs
Publication statusPublished - Aug 2010

Keywords

  • Animals Bacterial Typing Techniques Campylobacter Infections/*epidemiology/*microbiology Campylobacter coli/*classification/isolation & purification Campylobacter jejuni/*classification/isolation & purification Chickens/microbiology Cluster Analysis DNA Fingerprinting Genotype Geography Meat/*microbiology Molecular Epidemiology Netherlands/epidemiology Ruminants/microbiology Senegal/epidemiology Sequence Analysis, DNA Swine/microbiology United Kingdom/epidemiology United States/epidemiology

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