TY - JOUR
T1 - HT-SPOTi: A rapid drug susceptibility test (DST) to evaluate antibiotic resistance profiles and novel chemicals for anti-infective drug discovery
AU - Danquah, Cynthia A.
AU - Maitra, Arundhati
AU - Gibbons, Simon
AU - Faull, Jane
AU - Bhakta, Sanjib
PY - 2016/2
Y1 - 2016/2
N2 - Antibiotic resistance is one of the major threats to global health and wellbeing. The past decade has seen an alarming rise in the evolution and spread of drug-resistant strains of pathogenic microbes. The emergence of extensively drug resistant (XDR) strains of Mycobacterium tuberculosis and antimicrobial resistance among the ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, andEnterobacter species) as well as fungal pathogens (such as certain species of Candida, Aspergillus, Cryptococcus, and Trichophyton) poses a significant 21st century scientific challenge. With an extremely limited arsenal of efficacious antibiotics, techniques that can (a) identify novel antimicrobials and (b) detect antimicrobial resistance are becoming increasingly important. In this article, we illustrate the HT-SPOTi, an assay that is principally based on the growth of an organism on agar medium containing a range of different concentrations of drugs or inhibitors. The simple methodology makes this assay ideal for evaluating novel antimicrobial compounds as well as profiling an organism’s antibiotic resistance profile.
AB - Antibiotic resistance is one of the major threats to global health and wellbeing. The past decade has seen an alarming rise in the evolution and spread of drug-resistant strains of pathogenic microbes. The emergence of extensively drug resistant (XDR) strains of Mycobacterium tuberculosis and antimicrobial resistance among the ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, andEnterobacter species) as well as fungal pathogens (such as certain species of Candida, Aspergillus, Cryptococcus, and Trichophyton) poses a significant 21st century scientific challenge. With an extremely limited arsenal of efficacious antibiotics, techniques that can (a) identify novel antimicrobials and (b) detect antimicrobial resistance are becoming increasingly important. In this article, we illustrate the HT-SPOTi, an assay that is principally based on the growth of an organism on agar medium containing a range of different concentrations of drugs or inhibitors. The simple methodology makes this assay ideal for evaluating novel antimicrobial compounds as well as profiling an organism’s antibiotic resistance profile.
KW - Antimicrobial resistance
KW - Diagnosis
KW - Drug discovery
KW - Drug susceptibility testing (DST)
KW - HT-SPOTi
KW - Infectious diseases
KW - Whole-cell phenotypic evaluation
UR - http://www.scopus.com/inward/record.url?scp=84962151682&partnerID=8YFLogxK
U2 - 10.1002/9780471729259.mc1708s40
DO - 10.1002/9780471729259.mc1708s40
M3 - Article
C2 - 26855282
AN - SCOPUS:84962151682
SN - 1934-8525
VL - 2016
SP - 17.8.1-17.8.12
JO - Current Protocols in Microbiology
JF - Current Protocols in Microbiology
ER -