Hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a Pseudomonas species

Josa Marie Wehrfritz, Jon P. Carter, Stephen Spiro, David J. Richardson

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25 Citations (Scopus)

Abstract

Hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera Pseudomonas, Moraxella, Arthrobacter and Aeromonas. A hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the Pseudomonas and Aeromonas spp. and in total soluble fractions of the Arthrobacter sp. A monomeric 19-kDa non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the Pseudomonas species and shown to be similar to hydroxylamine-cytochrome c oxidoreductase of Paracoccus denitrificans.

Original languageEnglish
Pages (from-to)421-424
Number of pages4
JournalArchives of Microbiology
Volume166
Issue number6
DOIs
Publication statusPublished - 1996

Keywords

  • ammonia monooxygenase
  • denitrification
  • hydroxylamine-cytochrome c oxidoreductase
  • nitrification
  • Pseudomonas sp.

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