Identification of a novel botulinum neurotoxin gene cluster in Enterococcus

Jason Brunt; Andrew T. Carter; Sandra C Stringer; Michael W. Peck

doi:10.1002/1873-3468.12969

Identification of a novel botulinum neurotoxin gene cluster in Enterococcus

Jason Brunt, Andrew T. Carter, Sandra C Stringer, Michael W. Peck

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

78 Citations (Scopus)

34 Downloads (Pure)

Abstract

The deadly neurotoxins of Clostridium botulinum (BoNTs) comprise eight serotypes (A–G; X). The neurotoxin gene cluster encoding BoNT and its accessory proteins includes an operon containing an ntnh gene upstream of the boNT gene. Another operon contains either ha (haemagglutinin) or orfX genes (of unknown function). Here we describe a novel boNT gene cluster from Enterococcus sp. 3G1_DIV0629, with a typical ntnh gene and an uncommon orfX arrangement. The neurotoxin (designated putative eBoNT/J) contains a metallopeptidase zinc-binding site, a translocation domain and a target cell attachment domain. Structural properties of the latter suggest a novel targeting mechanism with consequent implications for application by the pharmaceutical industry. This is the first complete boNT gene cluster identified in a non-clostridial genome.

Original language	English
Pages (from-to)	310–317
Journal	FEBS Letters
Volume	592
Issue number	3
Early online date	23 Jan 2018
DOIs	https://doi.org/10.1002/1873-3468.12969 https://doi.org/10.1002/1873-3468.12969
Publication status	Published - Feb 2018

Keywords

botulinum neurotoxin
Clostridium
eBoNT/J
Enterococcus

Access to Document

10.1002/1873-3468.12969Licence: CC BY
10.1002/1873-3468.12969

Published manuscript
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 556 KBLicence: CC BY

http://doi.wiley.com/10.1002/1873-3468.12969Licence: CC BY

Cite this

@article{3b0f007a9aff4894a4ba17736a4dbfc6,

title = "Identification of a novel botulinum neurotoxin gene cluster in Enterococcus",

abstract = "The deadly neurotoxins of Clostridium botulinum (BoNTs) comprise eight serotypes (A–G; X). The neurotoxin gene cluster encoding BoNT and its accessory proteins includes an operon containing an ntnh gene upstream of the boNT gene. Another operon contains either ha (haemagglutinin) or orfX genes (of unknown function). Here we describe a novel boNT gene cluster from Enterococcus sp. 3G1_DIV0629, with a typical ntnh gene and an uncommon orfX arrangement. The neurotoxin (designated putative eBoNT/J) contains a metallopeptidase zinc-binding site, a translocation domain and a target cell attachment domain. Structural properties of the latter suggest a novel targeting mechanism with consequent implications for application by the pharmaceutical industry. This is the first complete boNT gene cluster identified in a non-clostridial genome.",

keywords = "botulinum neurotoxin, Clostridium, eBoNT/J, Enterococcus",

author = "Jason Brunt and Carter, {Andrew T.} and Stringer, {Sandra C} and Peck, {Michael W.}",

year = "2018",

month = feb,

doi = "10.1002/1873-3468.12969",

language = "English",

volume = "592",

pages = "310–317",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Identification of a novel botulinum neurotoxin gene cluster in Enterococcus

AU - Brunt, Jason

AU - Carter, Andrew T.

AU - Stringer, Sandra C

AU - Peck, Michael W.

PY - 2018/2

Y1 - 2018/2

N2 - The deadly neurotoxins of Clostridium botulinum (BoNTs) comprise eight serotypes (A–G; X). The neurotoxin gene cluster encoding BoNT and its accessory proteins includes an operon containing an ntnh gene upstream of the boNT gene. Another operon contains either ha (haemagglutinin) or orfX genes (of unknown function). Here we describe a novel boNT gene cluster from Enterococcus sp. 3G1_DIV0629, with a typical ntnh gene and an uncommon orfX arrangement. The neurotoxin (designated putative eBoNT/J) contains a metallopeptidase zinc-binding site, a translocation domain and a target cell attachment domain. Structural properties of the latter suggest a novel targeting mechanism with consequent implications for application by the pharmaceutical industry. This is the first complete boNT gene cluster identified in a non-clostridial genome.

AB - The deadly neurotoxins of Clostridium botulinum (BoNTs) comprise eight serotypes (A–G; X). The neurotoxin gene cluster encoding BoNT and its accessory proteins includes an operon containing an ntnh gene upstream of the boNT gene. Another operon contains either ha (haemagglutinin) or orfX genes (of unknown function). Here we describe a novel boNT gene cluster from Enterococcus sp. 3G1_DIV0629, with a typical ntnh gene and an uncommon orfX arrangement. The neurotoxin (designated putative eBoNT/J) contains a metallopeptidase zinc-binding site, a translocation domain and a target cell attachment domain. Structural properties of the latter suggest a novel targeting mechanism with consequent implications for application by the pharmaceutical industry. This is the first complete boNT gene cluster identified in a non-clostridial genome.

KW - botulinum neurotoxin

KW - Clostridium

KW - eBoNT/J

KW - Enterococcus

U2 - 10.1002/1873-3468.12969

DO - 10.1002/1873-3468.12969

M3 - Article

VL - 592

SP - 310

EP - 317

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -