Projects per year
i-Motifs are quadruplex DNA structures formed from sequences rich in cytosine and held together by intercalated, hemi-protonated cytosine–cytosine base pairs. These sequences are prevalent in gene promoter regions and may play a role in gene transcription. Targeting these structures with ligands could provide a novel way to target genetic disease but there are very few ligands which have been shown to interact with i-motif DNA. Fluorescent intercalator displacement (FID) assays are a simple way to screen ligands against DNA secondary structures. Here we characterise how thiazole orange interacts with i-motif DNA and assess its ability for use in a FID assay. Additionally, we report FID-based ligand screening using thiazole orange against the i-motif forming sequence from the human telomere to reveal new i-motif binding compounds which have the potential for further development.
|Number of pages||5|
|Journal||Organic & Biomolecular Chemistry|
|Early online date||1 Jun 2017|
|Publication status||Published - 21 Jul 2017|
- School of Pharmacy - Associate Professor
- School of Natural Sciences - Executive Member
Person: Member, Academic, Teaching & Research
- 1 Finished
Investigating the stability and function of i-motif DNA.
Biotechnology and Biological Sciences Research Council
30/11/14 → 29/12/17