Identification of SNAREs that mediate zymogen granule exocytosis

James A. Pickett, Manuel Campos-Toimil, Paul Thomas, J. Michael Edwardson

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20 Citations (Scopus)

Abstract

A secretagogue-stimulated pancreatic acinar cell releases digestive enzymes from its apical pole. We attempted to identify the SNAREs involved in zymogen granule exocytosis. Antibodies against syntaxins 2 and 3, SNAP-23 and VAMP 8, and the corresponding recombinant SNAREs, inhibited amylase secretion from streptolysin O-permeabilised acini; other anti-SNARE antibodies and SNAREs had no effect. Botulinum neurotoxin C, which cleaved syntaxin 2 and (to a lesser extent) syntaxin 3, but not syntaxins 4, 7 or 8, also inhibited exocytosis. We propose that syntaxin 2, SNAP-23 and VAMP 8 mediate primary granule–plasma membrane fusion. Syntaxin 3 may be involved in secondary granule–granule fusion.
Original languageEnglish
Pages (from-to)599-603
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume359
Issue number3
DOIs
Publication statusPublished - 3 Aug 2007

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