TY - JOUR
T1 - Identification of SNAREs that mediate zymogen granule exocytosis
AU - Pickett, James A.
AU - Campos-Toimil, Manuel
AU - Thomas, Paul
AU - Edwardson, J. Michael
PY - 2007/8/3
Y1 - 2007/8/3
N2 - A secretagogue-stimulated pancreatic acinar cell releases digestive enzymes from its apical pole. We attempted to identify the SNAREs involved in zymogen granule exocytosis. Antibodies against syntaxins 2 and 3, SNAP-23 and VAMP 8, and the corresponding recombinant SNAREs, inhibited amylase secretion from streptolysin O-permeabilised acini; other anti-SNARE antibodies and SNAREs had no effect. Botulinum neurotoxin C, which cleaved syntaxin 2 and (to a lesser extent) syntaxin 3, but not syntaxins 4, 7 or 8, also inhibited exocytosis. We propose that syntaxin 2, SNAP-23 and VAMP 8 mediate primary granule–plasma membrane fusion. Syntaxin 3 may be involved in secondary granule–granule fusion.
AB - A secretagogue-stimulated pancreatic acinar cell releases digestive enzymes from its apical pole. We attempted to identify the SNAREs involved in zymogen granule exocytosis. Antibodies against syntaxins 2 and 3, SNAP-23 and VAMP 8, and the corresponding recombinant SNAREs, inhibited amylase secretion from streptolysin O-permeabilised acini; other anti-SNARE antibodies and SNAREs had no effect. Botulinum neurotoxin C, which cleaved syntaxin 2 and (to a lesser extent) syntaxin 3, but not syntaxins 4, 7 or 8, also inhibited exocytosis. We propose that syntaxin 2, SNAP-23 and VAMP 8 mediate primary granule–plasma membrane fusion. Syntaxin 3 may be involved in secondary granule–granule fusion.
U2 - 10.1016/j.bbrc.2007.05.128
DO - 10.1016/j.bbrc.2007.05.128
M3 - Article
SN - 1090-2104
VL - 359
SP - 599
EP - 603
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -