Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation

Victoria Sherwood, Hans-Gerhard Burgert, Yun-Hsiang Chen, Sandeep Sanghera, Socrates Katafigiotis, Richard E. Randall, Ian Connerton, Kenneth H. Mellits

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    27 Citations (Scopus)

    Abstract

    Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.
    Original languageEnglish
    Pages (from-to)71-76
    Number of pages6
    JournalJournal of General Virology
    Volume88
    Issue number1
    DOIs
    Publication statusPublished - 2007

    Keywords

    • ACTIVATION
    • GASTROENTERITIS
    • SENSITIVITY
    • VIRUS-REPLICATION
    • CONTRIBUTE
    • PROMOTER
    • DEGRADATION
    • PROTEINS
    • SIMIAN-VIRUS-5
    • STAT1

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