Increased gelatinase A (MMP-2) and gelatinase B (MMP-9) activities in human brain after focal ischemia

Arthur W. Clark, Craig A. Krekoski, Shao-Sun Bou, Kevin R. Chapman, Dylan R. Edwards

Research output: Contribution to journalArticle

249 Citations (Scopus)

Abstract

Matrix metalloproteinases (MMPs) are involved in remodelling extracellular matrix. Gelatinase B (MMP-9) is an inducible 92 kDa MMP expressed by neutrophils, microglia, and endothelial cells. Gelatinase A (MMP-2) is a 72 kDa MMP, constitutively expressed in brain. Elevated MMP activity has been linked to various pathologic conditions, and the therapeutic benefit of MMP inhibitors is under study in a few experimental models. Using gelatin zymography, we have compared activities of these MMPs in infarcted and matched non-infarcted cerebral tissue from eight subjects dying at intervals of less than 2 h to several years after a stroke. Gelatinase B activity was markedly elevated in the infarcted tissue at two days post-infarction, and remained elevated in cases dying months after the event. Increases in gelatinase A activity were subtle at 2-5 days; they were marked and significant in cases dying at 4 months and later. The findings indicate distinct temporal profiles of post-ischemic gelatinase activity in human brain, with earlier but equally persistent elevation in gelatinase B when compared to gelatinase A.
Original languageEnglish
Pages (from-to)53-6
Number of pages4
JournalNeuroscience Letters
Volume238
Issue number1-2
DOIs
Publication statusPublished - 28 Nov 1997

Keywords

  • Aged
  • Aged, 80 and over
  • Brain Ischemia
  • Collagenases
  • Female
  • Gelatinases
  • Humans
  • Male
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9
  • Metalloendopeptidases
  • Middle Aged
  • Time Factors

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