TY - JOUR
T1 - Individual oligodendrocytes have only a few hours in which to generate new myelin sheaths invivo
AU - Czopka, Tim
AU - ffrench-Constant, Charles
AU - Lyons, David A.
N1 - Funding Information:
We thank Carl Tucker, Paul Wright, and Patricia Smart for fishroom support and Chris Jopling for information about the fyn MO. We would also like to thank Crerar hotels and Sally Womersley for helping to provide microscopes. A very special thanks goes to our late and great colleague Chi-Bin Chien and the Chien laboratory for the tol2kit and to Len Zon for the casper line. We are grateful to Peter Brophy, Ben Emery, Kelly Monk, William Talbot, and members of the Lyons and ffrench-Constant laboratories for critical reading of the manuscript. This work was supported by a David Phillips Fellowship from the BBSRC, a Research Prize from the Lister Institute, and an International Reintegration Grant (to D.A.L.), an EMBO Long-Term Fellowship (to T.C.), and a Wellcome Trust Programme Grant (to C.ff.-C.).
PY - 2013/6/24
Y1 - 2013/6/24
N2 - The number of myelin sheaths made by individual oligodendrocytes regulates the extent of myelination, which profoundly affects central nervous system function. It remains unknown when, during their life, individual oligodendrocytes can regulate myelin sheath number invivo. We show, using live imaging in zebrafish, that oligodendrocytes make new myelinsheaths during a period of just 5hr, with regulation of sheath number after this time limited to occasional retractions. We also show that activation and reduction of Fyn kinase in oligodendrocytes increases and decreases sheath number per cell, respectively. Interestingly, these oligodendrocytes also generate their new myelin sheaths within the same period, despite having vastly different extents of myelination. Our data demonstrate a restricted time window relative to the lifetime of the individual oligodendrocyte, during which myelin sheath formation occurs and the number of sheaths is determined.
AB - The number of myelin sheaths made by individual oligodendrocytes regulates the extent of myelination, which profoundly affects central nervous system function. It remains unknown when, during their life, individual oligodendrocytes can regulate myelin sheath number invivo. We show, using live imaging in zebrafish, that oligodendrocytes make new myelinsheaths during a period of just 5hr, with regulation of sheath number after this time limited to occasional retractions. We also show that activation and reduction of Fyn kinase in oligodendrocytes increases and decreases sheath number per cell, respectively. Interestingly, these oligodendrocytes also generate their new myelin sheaths within the same period, despite having vastly different extents of myelination. Our data demonstrate a restricted time window relative to the lifetime of the individual oligodendrocyte, during which myelin sheath formation occurs and the number of sheaths is determined.
UR - http://www.scopus.com/inward/record.url?scp=84879521914&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2013.05.013
DO - 10.1016/j.devcel.2013.05.013
M3 - Article
C2 - 23806617
AN - SCOPUS:84879521914
VL - 25
SP - 599
EP - 609
JO - Developmental Cell
JF - Developmental Cell
SN - 1534-5807
IS - 6
ER -