TY - JOUR
T1 - Influence of crude extract and bioactive fractions of Ilex paraguariensis A. St. Hil. (yerba mate) on the Wistar rat lipid metabolism
AU - de Resende, Pedro Ernesto
AU - Kaiser, Samuel
AU - Pittol, Vanessa
AU - Hoefel, Ana Lúcia
AU - D'Agostini Silva, Raquel
AU - Vieira Marques, Cláudia
AU - Kucharski, Luiz Carlos
AU - Ortega, George González
PY - 2015/5
Y1 - 2015/5
N2 - The influence of yerba mate extract (YME) and two concentrated fractions of methylxanthines–polyphenols (MXPL) and saponins (SFL) from yerba mate on the lipid metabolism of Wistar rats fed with a high-fat diet were examined. A Box–Behnken design was employed for the optimization of yerba mate extraction. Subsequently, one-step separation using solid phase fractionation was applied. HPLC–PDA and UPLC/Q-TOF-MS were used for quantification and identification of the main compounds. YME induced reductions on intra-abdominal fat weight gain and LDL plasma level. MXPL decreased plasma levels of cholesterol, LDL and triacylglycerols. In addition, this fraction increased lipogenesis of the muscle, hepatic glycogen synthesis and lipolysis in adipose tissue. SFL enhanced lipogenesis in adipose tissue and also fecal fat excretion comparing to both controls. Moreover, all mate preparations could entirely revert the hepatic lipogenesis induced by the high-fat diet. Thus, the yerba mate extract and fractions exhibited a potential anti-obesity effect on lipid metabolism of the Wistar rat and deserve further investigation.
AB - The influence of yerba mate extract (YME) and two concentrated fractions of methylxanthines–polyphenols (MXPL) and saponins (SFL) from yerba mate on the lipid metabolism of Wistar rats fed with a high-fat diet were examined. A Box–Behnken design was employed for the optimization of yerba mate extraction. Subsequently, one-step separation using solid phase fractionation was applied. HPLC–PDA and UPLC/Q-TOF-MS were used for quantification and identification of the main compounds. YME induced reductions on intra-abdominal fat weight gain and LDL plasma level. MXPL decreased plasma levels of cholesterol, LDL and triacylglycerols. In addition, this fraction increased lipogenesis of the muscle, hepatic glycogen synthesis and lipolysis in adipose tissue. SFL enhanced lipogenesis in adipose tissue and also fecal fat excretion comparing to both controls. Moreover, all mate preparations could entirely revert the hepatic lipogenesis induced by the high-fat diet. Thus, the yerba mate extract and fractions exhibited a potential anti-obesity effect on lipid metabolism of the Wistar rat and deserve further investigation.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84929239606&partnerID=MN8TOARS
U2 - 10.1016/j.jff.2015.03.040
DO - 10.1016/j.jff.2015.03.040
M3 - Article
VL - 15
SP - 440
EP - 451
JO - Journal of Functional Foods
JF - Journal of Functional Foods
SN - 1756-4646
ER -
