Inorganic nitrate, hypoxia, and the regulation of cardiac mitochondrial respiration - probing the role of PPARα

James A. Horscroft, Katie A. O'Brien, Anna D. Clark, Ross T. Lindsay, Alice Strang Steel, Nathan Procter, Jules Devaux, Michael Frenneaux, Stephen D. R. Harridge, Andrew Murray

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9 Citations (Scopus)
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Abstract

Dietary inorganic nitrate prevents aspects of cardiac mitochondrial dysfunction induced by hypoxia, although the mechanism is not completely understood. In both heart and skeletal muscle, nitrate increases fatty acid oxidation capacity, and in the latter case, this involves up-regulation of peroxisome proliferator-activated receptor (PPAR)α expression. Here, we investigated whether dietary nitrate modifies mitochondrial function in the hypoxic heart in a PPARα-dependent manner. Wild-type (WT) mice and mice without PPARα (Ppara−/−) were given water containing 0.7 mM NaCl (control) or 0.7 mM NaNO3 for 35 d. After 7 d, mice were exposed to normoxia or hypoxia (10% O2) for the remainder of the study. Mitochondrial respiratory function and metabolism were assessed in saponin-permeabilized cardiac muscle fibers. Environmental hypoxia suppressed mass-specific mitochondrial respiration and additionally lowered the proportion of respiration supported by fatty acid oxidation by 18% (P < 0.001). This switch away from fatty acid oxidation was reversed by nitrate treatment in hypoxic WT but not Ppara−/− mice, indicating a PPARα-dependent effect. Hypoxia increased hexokinase activity by 33% in all mice, whereas lactate dehydrogenase activity increased by 71% in hypoxic WT but not Ppara−/− mice. Our findings indicate that PPARα plays a key role in mediating cardiac metabolic remodeling in response to both hypoxia and dietary nitrate supplementation.
Original languageEnglish
Pages (from-to)7563-7577
Number of pages15
JournalThe FASEB Journal
Volume33
Issue number6
Early online date14 Mar 2019
DOIs
Publication statusPublished - Jun 2019

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