Instantaneous, stoichiometric generation of powerfully reducing states of protein active sites using Eu(II) and polyaminocarboxylate ligands

Kylie A. Vincent, Gareth J. Tilley, Nina C. Quammie, Ian Streeter, Barbara K. Burgess, Myles R. Cheesman, Fraser A. Armstrong

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Addition of an equivalent of a polyaminocarboxylate ligand (L) to a solution of a redox protein and the aqua Eu2+ ion results in the instantaneous in situ generation of a very powerful reductant Eu-II-L that can rapidly drive an electron stoichiometrically onto a redox centre having an extremely negative reduction potential ( lower than -1 V): this is exemplified by straightforward generation of the superreduced state of the Fe-protein of nitrogenase.
Original languageEnglish
Pages (from-to)2590-2591
Number of pages2
JournalChemical Communications
Issue number20
Publication statusPublished - 2003

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