Intraepithelial γδ+ Lymphocytes Maintain the Integrity of Intestinal Epithelial Tight Junctions in Response to Infection

Jane E Dalton, Sheena M Cruickshank, Charlotte E Egan, Rainy Mears, Darren J Newton, Elizabeth M Andrew, Beth Lawrence, Gareth Howell, Kathryn J Else, Marc-Jan Gubbels, Boris Striepen, Judith E Smith, Stanley J White, Simon R Carding

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)

Abstract

Intestinal epithelial integrity and permeability is dependent on intercellular tight junction (TJ) complexes. How TJ integrity is regulated remains unclear, although phosphorylation and dephosphorylation of the integral membrane protein occludin is an important determinant of TJ formation and epithelial permeability. We have investigated the role intestinal intraepithelial lymphocytes (iIELs) play in regulating epithelial permeability in response to infection.
Original languageEnglish
Pages (from-to)818-829
Number of pages12
JournalGastroenterology
Volume131
Issue number3
DOIs
Publication statusPublished - Sep 2006

Keywords

  • Animals
  • Blotting, Western
  • Cell Membrane Permeability
  • Disease Models, Animal
  • Immunohistochemistry
  • Immunoprecipitation
  • Intercellular Junctions
  • Intestinal Mucosa
  • Male
  • Membrane Proteins
  • Mice
  • Mice, Inbred C57BL
  • Occludin
  • Phosphorylation
  • RNA
  • Receptors, Antigen, T-Cell, gamma-delta
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes
  • Toxoplasma
  • Toxoplasmosis, Animal

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