Intraepithelial γδ+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection

Jane E Dalton; Sheena M Cruickshank; Charlotte E Egan; Rainy Mears; Darren J Newton; Elizabeth M Andrew; Beth Lawrence; Gareth Howell; Kathryn J Else; Marc-Jan Gubbels; Boris Striepen; Judith E Smith; Stanley J White; Simon R Carding

doi:10.1053/j.gastro.2006.06.003

Intraepithelial γδ+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection

Jane E Dalton, Sheena M Cruickshank, Charlotte E Egan, Rainy Mears, Darren J Newton, Elizabeth M Andrew, Beth Lawrence, Gareth Howell, Kathryn J Else, Marc-Jan Gubbels, Boris Striepen, Judith E Smith, Stanley J White, Simon R Carding

Research output: Contribution to journal › Article › peer-review

111 Citations (Scopus)

Abstract

Intestinal epithelial integrity and permeability is dependent on intercellular tight junction (TJ) complexes. How TJ integrity is regulated remains unclear, although phosphorylation and dephosphorylation of the integral membrane protein occludin is an important determinant of TJ formation and epithelial permeability. We have investigated the role intestinal intraepithelial lymphocytes (iIELs) play in regulating epithelial permeability in response to infection.

Original language	English
Pages (from-to)	818-829
Number of pages	12
Journal	Gastroenterology
Volume	131
Issue number	3
DOIs	https://doi.org/10.1053/j.gastro.2006.06.003
Publication status	Published - Sep 2006

Keywords

Animals
Blotting, Western
Cell Membrane Permeability
Disease Models, Animal
Immunohistochemistry
Immunoprecipitation
Intercellular Junctions
Intestinal Mucosa
Male
Membrane Proteins
Mice
Mice, Inbred C57BL
Occludin
Phosphorylation
RNA
Receptors, Antigen, T-Cell, gamma-delta
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes
Toxoplasma
Toxoplasmosis, Animal

Access to Document

10.1053/j.gastro.2006.06.003

Cite this

Dalton, J. E., Cruickshank, S. M., Egan, C. E., Mears, R., Newton, D. J., Andrew, E. M., Lawrence, B., Howell, G., Else, K. J., Gubbels, M-J., Striepen, B., Smith, J. E., White, S. J., & Carding, S. R. (2006). Intraepithelial γδ+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection. Gastroenterology, 131(3), 818-829. https://doi.org/10.1053/j.gastro.2006.06.003

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title = "Intraepithelial γδ+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection",

abstract = "Intestinal epithelial integrity and permeability is dependent on intercellular tight junction (TJ) complexes. How TJ integrity is regulated remains unclear, although phosphorylation and dephosphorylation of the integral membrane protein occludin is an important determinant of TJ formation and epithelial permeability. We have investigated the role intestinal intraepithelial lymphocytes (iIELs) play in regulating epithelial permeability in response to infection.",

keywords = "Animals, Blotting, Western, Cell Membrane Permeability, Disease Models, Animal, Immunohistochemistry, Immunoprecipitation, Intercellular Junctions, Intestinal Mucosa, Male, Membrane Proteins, Mice, Mice, Inbred C57BL, Occludin, Phosphorylation, RNA, Receptors, Antigen, T-Cell, gamma-delta, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes, Toxoplasma, Toxoplasmosis, Animal",

author = "Dalton, {Jane E} and Cruickshank, {Sheena M} and Egan, {Charlotte E} and Rainy Mears and Newton, {Darren J} and Andrew, {Elizabeth M} and Beth Lawrence and Gareth Howell and Else, {Kathryn J} and Marc-Jan Gubbels and Boris Striepen and Smith, {Judith E} and White, {Stanley J} and Carding, {Simon R}",

year = "2006",

month = sep,

doi = "10.1053/j.gastro.2006.06.003",

language = "English",

volume = "131",

pages = "818--829",

journal = "Gastroenterology",

issn = "0016-5085",

publisher = "Elsevier",

number = "3",

}

Dalton, JE, Cruickshank, SM, Egan, CE, Mears, R, Newton, DJ, Andrew, EM, Lawrence, B, Howell, G, Else, KJ, Gubbels, M-J, Striepen, B, Smith, JE, White, SJ & Carding, SR 2006, 'Intraepithelial γδ+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection', Gastroenterology, vol. 131, no. 3, pp. 818-829. https://doi.org/10.1053/j.gastro.2006.06.003

TY - JOUR

T1 - Intraepithelial γδ+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection

AU - Dalton, Jane E

AU - Cruickshank, Sheena M

AU - Egan, Charlotte E

AU - Mears, Rainy

AU - Newton, Darren J

AU - Andrew, Elizabeth M

AU - Lawrence, Beth

AU - Howell, Gareth

AU - Else, Kathryn J

AU - Gubbels, Marc-Jan

AU - Striepen, Boris

AU - Smith, Judith E

AU - White, Stanley J

AU - Carding, Simon R

PY - 2006/9

Y1 - 2006/9

N2 - Intestinal epithelial integrity and permeability is dependent on intercellular tight junction (TJ) complexes. How TJ integrity is regulated remains unclear, although phosphorylation and dephosphorylation of the integral membrane protein occludin is an important determinant of TJ formation and epithelial permeability. We have investigated the role intestinal intraepithelial lymphocytes (iIELs) play in regulating epithelial permeability in response to infection.

AB - Intestinal epithelial integrity and permeability is dependent on intercellular tight junction (TJ) complexes. How TJ integrity is regulated remains unclear, although phosphorylation and dephosphorylation of the integral membrane protein occludin is an important determinant of TJ formation and epithelial permeability. We have investigated the role intestinal intraepithelial lymphocytes (iIELs) play in regulating epithelial permeability in response to infection.

KW - Animals

KW - Blotting, Western

KW - Cell Membrane Permeability

KW - Disease Models, Animal

KW - Immunohistochemistry

KW - Immunoprecipitation

KW - Intercellular Junctions

KW - Intestinal Mucosa

KW - Male

KW - Membrane Proteins

KW - Mice

KW - Mice, Inbred C57BL

KW - Occludin

KW - Phosphorylation

KW - RNA

KW - Receptors, Antigen, T-Cell, gamma-delta

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - T-Lymphocytes

KW - Toxoplasma

KW - Toxoplasmosis, Animal

U2 - 10.1053/j.gastro.2006.06.003

DO - 10.1053/j.gastro.2006.06.003

M3 - Article

C2 - 16952551

VL - 131

SP - 818

EP - 829

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 3

ER -