@article{58a4d9d87d254b68af4022e4d6c4d8bc,
title = "Investigating the human intestinal DNA virome and predicting disease-associated virus–host interactions in severe myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)",
abstract = "Understanding how the human virome, and which of its constituents, contributes to health or disease states is reliant on obtaining comprehensive virome profiles. By combining DNA viromes from isolated virus-like particles (VLPs) and whole metagenomes from the same faecal sample of a small cohort of healthy individuals and patients with severe myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), we have obtained a more inclusive profile of the human intestinal DNA virome. Key features are the identification of a core virome comprising tailed phages of the class Caudoviricetes, and a greater diversity of DNA viruses including extracellular phages and integrated prophages. Using an in silico approach, we predicted interactions between members of the Anaerotruncus genus and unique viruses present in ME/CFS microbiomes. This study therefore provides a framework and rationale for studies of larger cohorts of patients to further investigate disease-associated interactions between the intestinal virome and the bacteriome.",
keywords = "bacteriome, bacteriophage, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), viral operational taxonomic unit (vOTU), virome, virus-like particle (VLP)",
author = "Hsieh, {Shen Yuan} and Savva, {George M.} and Andrea Telatin and Tiwari, {Sumeet K.} and Tariq, {Mohammad A.} and Fiona Newberry and Seton, {Katharine A.} and Catherine Booth and Bansal, {Amolak S.} and Thomas Wileman and Adriaenssens, {Evelien M.} and Carding, {Simon R.}",
note = "Data Availability Statement: The sequences derived from the VLP-enriched metagenomes have been deposited in ENA (https://www.ebi.ac.uk/ena/browser/home, accessed on 31 July 2023) under accession no. PRJEB57952 and sequences from the whole metagenomes (WMS) have also been deposited in ENA under accession no. PRJEB57953. Funding Information: This study was supported by the BBSRC Institute Strategic Programme Grant Gut Microbes and Health BB/R012490/1 and its constituent projects BBS/E/F/000PR10353, BBS/E/F/000PR10355 and BBS/E/F/000PR10356 (S.R.C., E.M.A, T.W.). The G.M.S. was funded by BBSRC Core Capability Grant BB/CCG1860/1. K.A.S. and F.N. were supported by PhD studentships jointly funded by the Invest in ME Research (UK Charity number 1153730) and the University of East Anglia. F.N. was funded by a Ramsey award from SOLVE M.E.",
year = "2023",
month = dec,
day = "8",
doi = "10.3390/ijms242417267",
language = "English",
volume = "24",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "MDPI AG",
number = "24",
}