iPSC-derived myelinoids to study myelin biology of humans

Owen G. James, Bhuvaneish T. Selvaraj, Dario Magnani, Karen Burr, Peter Connick, Samantha K. Barton, Navneet A. Vasistha, David W. Hampton, David Story, Robert Smigiel, Rafal Ploski, Peter J. Brophy, Charles ffrench-Constant, David A. Lyons, Siddharthan Chandran

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Myelination is essential for central nervous system (CNS) formation, health, and function. Emerging evidence of oligodendrocyte heterogeneity in health and disease and divergent CNS gene expression profiles between mice and humans supports the development of experimentally tractable human myelination systems. Here, we developed human iPSC-derived myelinating organoids (“myelinoids”) and quantitative tools to study myelination from oligodendrogenesis through to compact myelin formation and myelinated axon organization. Using patient-derived cells, we modeled a monogenetic disease of myelinated axons (Nfasc155 deficiency), recapitulating impaired paranodal axo-glial junction formation. We also validated the use of myelinoids for pharmacological assessment of myelination—both at the level of individual oligodendrocytes and globally across whole myelinoids—and demonstrated reduced myelination in response to suppressed synaptic vesicle release. Our study provides a platform to investigate human myelin development, disease, and adaptive myelination.

Original languageEnglish
Pages (from-to)1346-1358.e6
JournalDevelopmental Cell
Volume56
Issue number9
DOIs
Publication statusPublished - 3 May 2021

Keywords

  • adaptive myelination
  • disease modelling
  • human myelination
  • human stem cell
  • iPSC
  • myelin
  • myelinoid
  • oligodendrocyte
  • organoid
  • paranode

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