KazrinA is required for axial elongation and epidermal integrity in Xenopus tropicalis

Lisa M. Sevilla, Amer A. Rana, Fiona M. Watt, James C. Smith

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Kazrin is a recently described desmosomal protein that binds the cornified envelope precursor periplakin. In this study, we have examined kazrin isoform A expression during the development of Xenopus tropicalis and investigated the consequences of its depletion. Whole mount in situ hybridisation revealed that kazrin A mRNA is expressed throughout the embryo at least until tadpole stages. Xenopus tropicalis embryos that had been injected with antisense morpholino oligonucleotides directed against kazrinA failed to elongate properly and showed defects in development of the head, eye, notochord, and somites. We also observed that the epidermis became disorganised and frequently separated from the underlying mesoderm, causing the formation of epidermal blisters. Together, our results suggest that loss of kazrinA causes defects in cell adhesion that affect axial elongation, cell differentiation, and epidermal morphogenesis.

Original languageEnglish
Pages (from-to)1718-1725
Number of pages8
JournalDevelopmental Dynamics
Volume237
Issue number6
DOIs
Publication statusPublished - Jun 2008

Keywords

  • Antisense morpholino oligonucleotides
  • Cytoskeleton
  • Epidermis
  • Kazrin
  • Morphogenesis
  • Xenopus tropicalis

Cite this