Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin

Nick E. Le Brun; Michael T. Wilson; Simon C. Andrews; John R. Guest; Pauline M. Harrison; Andrew J. Thomson; Geoffrey R. Moore

doi:10.1016/0014-5793(93)80404-I

Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin

Nick E. Le Brun, Michael T. Wilson, Simon C. Andrews, John R. Guest, Pauline M. Harrison, Andrew J. Thomson, Geoffrey R. Moore

Research output: Contribution to journal › Article › peer-review

Abstract

The mechanism by which iron-storage proteins take up and oxidise iron(II) is not understood. We show by rapid-kinetic and EPR measurements that iron uptake, in vitro, by a bacterial iron-storage protein, bacterioferritin, involves at least three kinetically distinguishable phases: phase 1, the binding of Fe(II) ions, probably at a dimeric iron ferroxidase centre; phase 2, oxidation of the Fe(II) dimer and production of mononuclear Fe(III); and phase 3, iron core formation.

Original language	English
Pages (from-to)	197-202
Number of pages	6
Journal	FEBS Letters
Volume	333
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(93)80404-I
Publication status	Published - 25 Oct 1993

Keywords

Bacterioferritin
Iron(II) dimer
Iron-uptake
Kinetic phase

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10.1016/0014-5793(93)80404-I

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title = "Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin",

abstract = "The mechanism by which iron-storage proteins take up and oxidise iron(II) is not understood. We show by rapid-kinetic and EPR measurements that iron uptake, in vitro, by a bacterial iron-storage protein, bacterioferritin, involves at least three kinetically distinguishable phases: phase 1, the binding of Fe(II) ions, probably at a dimeric iron ferroxidase centre; phase 2, oxidation of the Fe(II) dimer and production of mononuclear Fe(III); and phase 3, iron core formation.",

keywords = "Bacterioferritin, Iron(II) dimer, Iron-uptake, Kinetic phase",

author = "{Le Brun}, {Nick E.} and Wilson, {Michael T.} and Andrews, {Simon C.} and Guest, {John R.} and Harrison, {Pauline M.} and Thomson, {Andrew J.} and Moore, {Geoffrey R.}",

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T1 - Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin

AU - Le Brun, Nick E.

AU - Wilson, Michael T.

AU - Andrews, Simon C.

AU - Guest, John R.

AU - Harrison, Pauline M.

AU - Thomson, Andrew J.

AU - Moore, Geoffrey R.

PY - 1993/10/25

Y1 - 1993/10/25

N2 - The mechanism by which iron-storage proteins take up and oxidise iron(II) is not understood. We show by rapid-kinetic and EPR measurements that iron uptake, in vitro, by a bacterial iron-storage protein, bacterioferritin, involves at least three kinetically distinguishable phases: phase 1, the binding of Fe(II) ions, probably at a dimeric iron ferroxidase centre; phase 2, oxidation of the Fe(II) dimer and production of mononuclear Fe(III); and phase 3, iron core formation.

AB - The mechanism by which iron-storage proteins take up and oxidise iron(II) is not understood. We show by rapid-kinetic and EPR measurements that iron uptake, in vitro, by a bacterial iron-storage protein, bacterioferritin, involves at least three kinetically distinguishable phases: phase 1, the binding of Fe(II) ions, probably at a dimeric iron ferroxidase centre; phase 2, oxidation of the Fe(II) dimer and production of mononuclear Fe(III); and phase 3, iron core formation.

KW - Bacterioferritin

KW - Iron(II) dimer

KW - Iron-uptake

KW - Kinetic phase

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DO - 10.1016/0014-5793(93)80404-I

M3 - Article

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AN - SCOPUS:0027430148

SN - 0014-5793

VL - 333

SP - 197

EP - 202

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin

Abstract

Keywords

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