Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin

Nick E. Le Brun; Michael T. Wilson; Simon C. Andrews; John R. Guest; Pauline M. Harrison; Andrew J. Thomson; Geoffrey R. Moore

doi:10.1016/0014-5793(93)80404-I

Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin

Nick E. Le Brun
, Michael T. Wilson
, Simon C. Andrews
, John R. Guest
, Pauline M. Harrison
, Andrew J. Thomson
, Geoffrey R. Moore

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

The mechanism by which iron-storage proteins take up and oxidise iron(II) is not understood. We show by rapid-kinetic and EPR measurements that iron uptake, in vitro, by a bacterial iron-storage protein, bacterioferritin, involves at least three kinetically distinguishable phases: phase 1, the binding of Fe(II) ions, probably at a dimeric iron ferroxidase centre; phase 2, oxidation of the Fe(II) dimer and production of mononuclear Fe(III); and phase 3, iron core formation.

Original language	English
Pages (from-to)	197-202
Number of pages	6
Journal	FEBS Letters
Volume	333
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(93)80404-I
Publication status	Published - 25 Oct 1993

Keywords

Bacterioferritin
Iron(II) dimer
Iron-uptake
Kinetic phase

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10.1016/0014-5793(93)80404-I

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title = "Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin",

abstract = "The mechanism by which iron-storage proteins take up and oxidise iron(II) is not understood. We show by rapid-kinetic and EPR measurements that iron uptake, in vitro, by a bacterial iron-storage protein, bacterioferritin, involves at least three kinetically distinguishable phases: phase 1, the binding of Fe(II) ions, probably at a dimeric iron ferroxidase centre; phase 2, oxidation of the Fe(II) dimer and production of mononuclear Fe(III); and phase 3, iron core formation.",

keywords = "Bacterioferritin, Iron(II) dimer, Iron-uptake, Kinetic phase",

author = "\{Le Brun\}, \{Nick E.\} and Wilson, \{Michael T.\} and Andrews, \{Simon C.\} and Guest, \{John R.\} and Harrison, \{Pauline M.\} and Thomson, \{Andrew J.\} and Moore, \{Geoffrey R.\}",

year = "1993",

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doi = "10.1016/0014-5793(93)80404-I",

language = "English",

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TY - JOUR

T1 - Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin

AU - Le Brun, Nick E.

AU - Wilson, Michael T.

AU - Andrews, Simon C.

AU - Guest, John R.

AU - Harrison, Pauline M.

AU - Thomson, Andrew J.

AU - Moore, Geoffrey R.

PY - 1993/10/25

Y1 - 1993/10/25

N2 - The mechanism by which iron-storage proteins take up and oxidise iron(II) is not understood. We show by rapid-kinetic and EPR measurements that iron uptake, in vitro, by a bacterial iron-storage protein, bacterioferritin, involves at least three kinetically distinguishable phases: phase 1, the binding of Fe(II) ions, probably at a dimeric iron ferroxidase centre; phase 2, oxidation of the Fe(II) dimer and production of mononuclear Fe(III); and phase 3, iron core formation.

AB - The mechanism by which iron-storage proteins take up and oxidise iron(II) is not understood. We show by rapid-kinetic and EPR measurements that iron uptake, in vitro, by a bacterial iron-storage protein, bacterioferritin, involves at least three kinetically distinguishable phases: phase 1, the binding of Fe(II) ions, probably at a dimeric iron ferroxidase centre; phase 2, oxidation of the Fe(II) dimer and production of mononuclear Fe(III); and phase 3, iron core formation.

KW - Bacterioferritin

KW - Iron(II) dimer

KW - Iron-uptake

KW - Kinetic phase

UR - https://www.scopus.com/pages/publications/0027430148

U2 - 10.1016/0014-5793(93)80404-I

DO - 10.1016/0014-5793(93)80404-I

M3 - Article

C2 - 8224163

AN - SCOPUS:0027430148

SN - 0014-5793

VL - 333

SP - 197

EP - 202

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin

Abstract

Keywords

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