Klebsiella pneumoniae isolate from South Africa with multiple TEM, SHV and AmpC beta-lactamases

Sabiha Y Essack, Lucinda M C Hall, David M Livermore

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Klebsiella pneumoniae 2207, from Durban, was resistant to cefoxitin and beta-lactamase inhibitor combinations as well as oxyimino-aminothiazolyl cephalosporins. Beta-lactamases with isoelectric points of 5.4, 5.6, 7.6, 8.2 and 8.4 were found. DNA hybridisation identified two BamHI and three HindIII fragments carrying blaTEM, and two SalI fragments carrying blaSHV. At least two genes encoded TEM-1 enzyme; one blaSHV copy encoded SHV-5 but the other determined SHV-23, a novel SHV-5 variant with conservative amino-acid substitutions far from the catalytic site. The pI 8.4 activity was an AmpC-type enzyme. Determinants of the pI 5.6 and 7.6 activities were not identified.
Original languageEnglish
Pages (from-to)398-400
Number of pages3
JournalInternational Journal of Antimicrobial Agents
Issue number4
Publication statusPublished - Apr 2004


  • Bacterial Proteins
  • Humans
  • Isoelectric Focusing
  • Klebsiella Infections
  • Klebsiella pneumoniae
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • South Africa
  • beta-Lactam Resistance
  • beta-Lactamases
  • beta-Lactams

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