Klebsiella pneumoniae isolate from South Africa with multiple TEM, SHV and AmpC beta-lactamases

Sabiha Y Essack; Lucinda M C Hall; David M Livermore

doi:10.1016/j.ijantimicag.2003.08.010

Klebsiella pneumoniae isolate from South Africa with multiple TEM, SHV and AmpC beta-lactamases

Sabiha Y Essack, Lucinda M C Hall, David M Livermore

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Klebsiella pneumoniae 2207, from Durban, was resistant to cefoxitin and beta-lactamase inhibitor combinations as well as oxyimino-aminothiazolyl cephalosporins. Beta-lactamases with isoelectric points of 5.4, 5.6, 7.6, 8.2 and 8.4 were found. DNA hybridisation identified two BamHI and three HindIII fragments carrying blaTEM, and two SalI fragments carrying blaSHV. At least two genes encoded TEM-1 enzyme; one blaSHV copy encoded SHV-5 but the other determined SHV-23, a novel SHV-5 variant with conservative amino-acid substitutions far from the catalytic site. The pI 8.4 activity was an AmpC-type enzyme. Determinants of the pI 5.6 and 7.6 activities were not identified.

Original language	English
Pages (from-to)	398-400
Number of pages	3
Journal	International Journal of Antimicrobial Agents
Volume	23
Issue number	4
DOIs	https://doi.org/10.1016/j.ijantimicag.2003.08.010
Publication status	Published - Apr 2004

Keywords

Bacterial Proteins
Humans
Isoelectric Focusing
Klebsiella Infections
Klebsiella pneumoniae
Microbial Sensitivity Tests
Molecular Sequence Data
Sequence Analysis, DNA
South Africa
beta-Lactam Resistance
beta-Lactamases
beta-Lactams

Access to Document

10.1016/j.ijantimicag.2003.08.010

Cite this

@article{3f8847a83262441199e083f4bb5b5361,

title = "Klebsiella pneumoniae isolate from South Africa with multiple TEM, SHV and AmpC beta-lactamases",

abstract = "Klebsiella pneumoniae 2207, from Durban, was resistant to cefoxitin and beta-lactamase inhibitor combinations as well as oxyimino-aminothiazolyl cephalosporins. Beta-lactamases with isoelectric points of 5.4, 5.6, 7.6, 8.2 and 8.4 were found. DNA hybridisation identified two BamHI and three HindIII fragments carrying blaTEM, and two SalI fragments carrying blaSHV. At least two genes encoded TEM-1 enzyme; one blaSHV copy encoded SHV-5 but the other determined SHV-23, a novel SHV-5 variant with conservative amino-acid substitutions far from the catalytic site. The pI 8.4 activity was an AmpC-type enzyme. Determinants of the pI 5.6 and 7.6 activities were not identified.",

keywords = "Bacterial Proteins, Humans, Isoelectric Focusing, Klebsiella Infections, Klebsiella pneumoniae, Microbial Sensitivity Tests, Molecular Sequence Data, Sequence Analysis, DNA, South Africa, beta-Lactam Resistance, beta-Lactamases, beta-Lactams",

author = "Essack, {Sabiha Y} and Hall, {Lucinda M C} and Livermore, {David M}",

year = "2004",

month = apr,

doi = "10.1016/j.ijantimicag.2003.08.010",

language = "English",

volume = "23",

pages = "398--400",

journal = "International Journal of Antimicrobial Agents",

issn = "0924-8579",

publisher = "Elsevier",

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TY - JOUR

T1 - Klebsiella pneumoniae isolate from South Africa with multiple TEM, SHV and AmpC beta-lactamases

AU - Essack, Sabiha Y

AU - Hall, Lucinda M C

AU - Livermore, David M

PY - 2004/4

Y1 - 2004/4

N2 - Klebsiella pneumoniae 2207, from Durban, was resistant to cefoxitin and beta-lactamase inhibitor combinations as well as oxyimino-aminothiazolyl cephalosporins. Beta-lactamases with isoelectric points of 5.4, 5.6, 7.6, 8.2 and 8.4 were found. DNA hybridisation identified two BamHI and three HindIII fragments carrying blaTEM, and two SalI fragments carrying blaSHV. At least two genes encoded TEM-1 enzyme; one blaSHV copy encoded SHV-5 but the other determined SHV-23, a novel SHV-5 variant with conservative amino-acid substitutions far from the catalytic site. The pI 8.4 activity was an AmpC-type enzyme. Determinants of the pI 5.6 and 7.6 activities were not identified.

AB - Klebsiella pneumoniae 2207, from Durban, was resistant to cefoxitin and beta-lactamase inhibitor combinations as well as oxyimino-aminothiazolyl cephalosporins. Beta-lactamases with isoelectric points of 5.4, 5.6, 7.6, 8.2 and 8.4 were found. DNA hybridisation identified two BamHI and three HindIII fragments carrying blaTEM, and two SalI fragments carrying blaSHV. At least two genes encoded TEM-1 enzyme; one blaSHV copy encoded SHV-5 but the other determined SHV-23, a novel SHV-5 variant with conservative amino-acid substitutions far from the catalytic site. The pI 8.4 activity was an AmpC-type enzyme. Determinants of the pI 5.6 and 7.6 activities were not identified.

KW - Bacterial Proteins

KW - Humans

KW - Isoelectric Focusing

KW - Klebsiella Infections

KW - Klebsiella pneumoniae

KW - Microbial Sensitivity Tests

KW - Molecular Sequence Data

KW - Sequence Analysis, DNA

KW - South Africa

KW - beta-Lactam Resistance

KW - beta-Lactamases

KW - beta-Lactams

U2 - 10.1016/j.ijantimicag.2003.08.010

DO - 10.1016/j.ijantimicag.2003.08.010

M3 - Article

C2 - 15081091

VL - 23

SP - 398

EP - 400

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

IS - 4

ER -