Lab-scale preparation and QC of phytase assay substrate from rice bran

Claus Madsen, Charles Brearley, Henrik Brinch-Pedersen

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Abstract

Sodium phytate is the most commonly used substrate for phytase assays. The current data documents the composition of sodium phytate prepared from rice bran on the lab scale. Two batches (A and B) of rice bran sodium phytate were analyzed for their elemental composition by ICP-SFMS. The preparation of the two batches differed by the introduction of an extra step to reduce contaminating magnesium for batch B. Both batches and additional samples of commercial and recrystallized commercial sodium phytate were also analyzed for their inositol phosphate profile by anion exchange HPLC.
Original languageEnglish
Pages (from-to)7-12
JournalAnalytical Biochemistry
Volume578
Early online date2 May 2019
DOIs
Publication statusPublished - 1 Aug 2019

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