Lab-scale preparation and QC of phytase assay substrate from rice bran

Claus Madsen; Charles Brearley; Henrik Brinch-Pedersen

doi:10.1016/j.ab.2019.04.021

Lab-scale preparation and QC of phytase assay substrate from rice bran

Claus Madsen, Charles Brearley, Henrik Brinch-Pedersen

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19 Citations (Scopus)

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Abstract

Sodium phytate is the most commonly used substrate for phytase assays. The current data documents the composition of sodium phytate prepared from rice bran on the lab scale. Two batches (A and B) of rice bran sodium phytate were analyzed for their elemental composition by ICP-SFMS. The preparation of the two batches differed by the introduction of an extra step to reduce contaminating magnesium for batch B. Both batches and additional samples of commercial and recrystallized commercial sodium phytate were also analyzed for their inositol phosphate profile by anion exchange HPLC.

Original language	English
Pages (from-to)	7-12
Number of pages	6
Journal	Analytical Biochemistry
Volume	578
Early online date	2 May 2019
DOIs	https://doi.org/10.1016/j.ab.2019.04.021
Publication status	Published - 1 Aug 2019

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10.1016/j.ab.2019.04.021

Accepted_ManuscriptAccepted author manuscript, 901 KB

Charles Brearley
- C.Brearleyuea.acuk
- School of Biological Sciences - Professor of Biochemistry
- Molecular Microbiology - Member
- Plant Sciences - Member
Person: Research Group Member, Academic, Teaching and Research

Phytases beyond phosphate, how inositol improves Feed Conversion ratio in poultry
Brearley, C. & Hajihosseini, M. K.
Biotechnology and Biological Sciences Research Council
27/06/16 → 26/11/18
Project: Research

Cite this

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title = "Lab-scale preparation and QC of phytase assay substrate from rice bran",

abstract = "Sodium phytate is the most commonly used substrate for phytase assays. The current data documents the composition of sodium phytate prepared from rice bran on the lab scale. Two batches (A and B) of rice bran sodium phytate were analyzed for their elemental composition by ICP-SFMS. The preparation of the two batches differed by the introduction of an extra step to reduce contaminating magnesium for batch B. Both batches and additional samples of commercial and recrystallized commercial sodium phytate were also analyzed for their inositol phosphate profile by anion exchange HPLC.",

author = "Claus Madsen and Charles Brearley and Henrik Brinch-Pedersen",

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AU - Madsen, Claus

AU - Brearley, Charles

AU - Brinch-Pedersen, Henrik

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Y1 - 2019/8/1

N2 - Sodium phytate is the most commonly used substrate for phytase assays. The current data documents the composition of sodium phytate prepared from rice bran on the lab scale. Two batches (A and B) of rice bran sodium phytate were analyzed for their elemental composition by ICP-SFMS. The preparation of the two batches differed by the introduction of an extra step to reduce contaminating magnesium for batch B. Both batches and additional samples of commercial and recrystallized commercial sodium phytate were also analyzed for their inositol phosphate profile by anion exchange HPLC.

AB - Sodium phytate is the most commonly used substrate for phytase assays. The current data documents the composition of sodium phytate prepared from rice bran on the lab scale. Two batches (A and B) of rice bran sodium phytate were analyzed for their elemental composition by ICP-SFMS. The preparation of the two batches differed by the introduction of an extra step to reduce contaminating magnesium for batch B. Both batches and additional samples of commercial and recrystallized commercial sodium phytate were also analyzed for their inositol phosphate profile by anion exchange HPLC.

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DO - 10.1016/j.ab.2019.04.021

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VL - 578

SP - 7

EP - 12

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

Lab-scale preparation and QC of phytase assay substrate from rice bran

Abstract

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Charles Brearley

Projects

Phytases beyond phosphate, how inositol improves Feed Conversion ratio in poultry

Cite this