Abstract
1. Organic forms of phosphorus (P) prevail in soils and their quantification is vital to better understand global biogeochemical cycles. P speciation in soil is commonly assessed by 31P NMR spectroscopy of sodium hydroxide-EDTA (NaOH-EDTA) extracts.
3. A liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) method that employs NaOH-EDTA is described.
3. Comparison with 31P NMR shows that LC-ICP-MS is up to three orders of magnitude more sensitive. It allows measurement in samples as small as 1 mg. We reveal variation of inositol phosphate distribution in Swedish boreal forest soil and identify myo- and scyllo-inositol hexakisphosphates and other isomers including scyllo-inositol pentakisphosphate.
4. Speciation of the major inositol phosphates was not altered by long-term nitrogen fertilization.
3. A liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) method that employs NaOH-EDTA is described.
3. Comparison with 31P NMR shows that LC-ICP-MS is up to three orders of magnitude more sensitive. It allows measurement in samples as small as 1 mg. We reveal variation of inositol phosphate distribution in Swedish boreal forest soil and identify myo- and scyllo-inositol hexakisphosphates and other isomers including scyllo-inositol pentakisphosphate.
4. Speciation of the major inositol phosphates was not altered by long-term nitrogen fertilization.
| Original language | English |
|---|---|
| Pages (from-to) | 530-543 |
| Number of pages | 14 |
| Journal | Methods in Ecology and Evolution |
| Volume | 15 |
| Issue number | 3 |
| Early online date | 4 Feb 2024 |
| DOIs | |
| Publication status | Published - Mar 2024 |
Keywords
- LC-ICP-MS
- inositol phosphate isomers
- nitrogen fertilization
- phosphorus speciation
- phytate
- soil 31P NMR
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