Lipid, detergent, and coomassie blue G-250 affect the migration of small membrane proteins in blue native gels: Mitochondrial carriers migrate as monomers not dimers

Paul G. Crichton, Marilyn Harding, Jonathan J. Ruprecht, Yang Lee, Edmund R. S. Kunji

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Abstract

Background: Mitochondrial carriers were thought to be dimeric based on their migration in blue native gels. 

Results: The high molecular mass species observed in blue native gels are composed of protein monomers, detergent, lipid, and Coomassie stain. 

Conclusion: The mitochondrial carriers are monomeric not dimeric. 

Significance: The apparent mass of small membrane proteins in blue native gels requires significant correction.

Original languageEnglish
Pages (from-to)22163-22173
Number of pages11
JournalJournal of Biological Chemistry
Volume288
Issue number30
Early online date6 Jun 2013
DOIs
Publication statusPublished - 26 Jul 2013

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