TY - JOUR
T1 - Lipid, detergent, and coomassie blue G-250 affect the migration of small membrane proteins in blue native gels
T2 - Mitochondrial carriers migrate as monomers not dimers
AU - Crichton, Paul G.
AU - Harding, Marilyn
AU - Ruprecht, Jonathan J.
AU - Lee, Yang
AU - Kunji, Edmund R. S.
N1 - Creative Commons: CC-BY license
PY - 2013/7/26
Y1 - 2013/7/26
N2 - Background: Mitochondrial carriers were thought to be dimeric based on their migration in blue native gels. Results: The high molecular mass species observed in blue native gels are composed of protein monomers, detergent, lipid, and Coomassie stain. Conclusion: The mitochondrial carriers are monomeric not dimeric. Significance: The apparent mass of small membrane proteins in blue native gels requires significant correction.
AB - Background: Mitochondrial carriers were thought to be dimeric based on their migration in blue native gels. Results: The high molecular mass species observed in blue native gels are composed of protein monomers, detergent, lipid, and Coomassie stain. Conclusion: The mitochondrial carriers are monomeric not dimeric. Significance: The apparent mass of small membrane proteins in blue native gels requires significant correction.
UR - http://www.scopus.com/inward/record.url?scp=84881237077&partnerID=8YFLogxK
U2 - 10.1074/jbc.M113.484329
DO - 10.1074/jbc.M113.484329
M3 - Article
C2 - 23744064
AN - SCOPUS:84881237077
VL - 288
SP - 22163
EP - 22173
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 30
ER -