Lipid-quantum dot bilayer vesicles enhance tumor cell uptake and retention in vitro and in vivo

Wafa' T Al-Jamal, Khuloud T Al-Jamal, Bowen Tian, Lara Lacerda, Paul H Bomans, Peter M Frederik, Kostas Kostarelos

Research output: Contribution to journalArticlepeer-review

124 Citations (Scopus)

Abstract

We report the construction of lipid-quantum dot (L-QD) bilayer vesicles by incorporation of the smallest (2 nm core size) commercially available CdSe/ZnS QD within zwitterionic dioleoylphosphatidylcholine and cationic 1,2-dioleoyl-3-trimethylammonium-propane lipid bilayers, self-assembling into small unilamellar vesicles. The incorporation of QD in the acyl environment of the lipid bilayer led to significant enhancement of their optical stability during storage and exposure to UV irradiation compared to that of QD alone in toluene. Moreover, structural characterization of L-QD hybrid bilayer vesicles using cryogenic electron microscopy revealed that the incorporation of QD takes place by hydrophobic self-association within the biomembranes. The L-QD vesicles bound and internalized in human epithelial lung cells (A549), and confocal laser scanning microscopy studies indicated that the L-QD were able to intracellularly traffick inside the cells. Moreover, cationic L-QD vesicles were injected in vivo intratumorally, leading to enhanced retention within human cervical carcinoma (C33a) xenografts. The hybrid L-QD bilayer vesicles presented here are thought to constitute a novel delivery system that offers the potential for transport of combinatory therapeutic and diagnostic modalities to cancer cells in vitro and in vivo.
Original languageEnglish
Pages (from-to)408-418
Number of pages11
JournalACS Nano
Volume2
Issue number3
DOIs
Publication statusPublished - Mar 2008

Keywords

  • Animals
  • Quantum Dots
  • Humans
  • Metabolic Clearance Rate
  • Cell Line, Tumor
  • Mice
  • Mice, Nude
  • Tissue Distribution
  • Uterine Cervical Neoplasms
  • Lung Neoplasms
  • Lipid Bilayers
  • Drug Carriers
  • Female

Cite this