TY - JOUR
T1 - Liquid Phase Electron Microscopy of Bacterial Ultrastructure
AU - Caffrey, Brian J.
AU - Pedrazo-Tardajos, Adrián
AU - Liberti, Emanuela
AU - Gaunt, Benjamin
AU - Kim, Judy S.
AU - Kirkland, Angus I.
N1 - Data Availability Statement:
The data that support the findings of this study are openly available in Zenodo at 10.5281/zenodo.10625794 and 10.5281/zenodo.11527417..
PY - 2024/12/12
Y1 - 2024/12/12
N2 - Recent advances in liquid phase scanning transmission electron microscopy (LP-STEM) have enabled the study of dynamic biological processes at nanometer resolutions, paving the way for live-cell imaging using electron microscopy. However, this technique is often hampered by the inherent thickness of whole cell samples and damage from electron beam irradiation. These restrictions degrade image quality and resolution, impeding biological interpretation. Using graphene encapsulation, scanning transmission electron microscopy (STEM), and energy-dispersive X-ray (EDX) spectroscopy to mitigate these issues provides unprecedented levels of intracellular detail in aqueous specimens. This study demonstrates the potential of LP-STEM to examine and identify internal cellular structures in thick biological samples. Specifically, it highlights the use of LP-STEM to investigate the radiation resistant, gram-positive bacterium, Deinococcus radiodurans using various imaging techniques.
AB - Recent advances in liquid phase scanning transmission electron microscopy (LP-STEM) have enabled the study of dynamic biological processes at nanometer resolutions, paving the way for live-cell imaging using electron microscopy. However, this technique is often hampered by the inherent thickness of whole cell samples and damage from electron beam irradiation. These restrictions degrade image quality and resolution, impeding biological interpretation. Using graphene encapsulation, scanning transmission electron microscopy (STEM), and energy-dispersive X-ray (EDX) spectroscopy to mitigate these issues provides unprecedented levels of intracellular detail in aqueous specimens. This study demonstrates the potential of LP-STEM to examine and identify internal cellular structures in thick biological samples. Specifically, it highlights the use of LP-STEM to investigate the radiation resistant, gram-positive bacterium, Deinococcus radiodurans using various imaging techniques.
KW - deinococcus.radiodurans
KW - energy dispersive x-ray spectroscopy
KW - graphene encapsulation
KW - liquid phase
KW - manganese uptake
KW - radiation resistance
KW - scanning transmission electron microscopy
UR - https://www.scopus.com/pages/publications/85203088538
U2 - 10.1002/smll.202402871
DO - 10.1002/smll.202402871
M3 - Article
C2 - 39239997
AN - SCOPUS:85203088538
SN - 1613-6810
VL - 20
JO - Small
JF - Small
IS - 50
M1 - 2402871
ER -