LUCA-I5-encoded sequence variants regulate CD95-mediated apoptosis

Leslie C. Sutherland; Sandra E. Edwards; Hazel C. Cable; Guy G. Poirier; Barbara A. Miller; Colin S. Cooper; Gwyn T. Williams

doi:10.1038/sj.onc.1203720

LUCA-I5-encoded sequence variants regulate CD95-mediated apoptosis

Leslie C. Sutherland, Sandra E. Edwards, Hazel C. Cable, Guy G. Poirier, Barbara A. Miller, Colin S. Cooper, Gwyn T. Williams

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Using an expression cloning system to discover novel genes involved in apoptosis, we identified a 326 bp bone marrow cDNA fragment (termed Je2) that suppresses, upon transfection, CD95-mediated apoptosis in Jurkat T cells. Sequence homology revealed that Je2 maps to 3p21.3, to an intronic region of the candidate TSG LUCA-15 locus. It represents, in fact, an antisense transcript to the 3'-UTR of two novel splice variants of this gene. Overexpression of sequence representing one of these splice variants (a 2.6 kb cDNA termed Clone 26), inhibited proliferation of Jurkat cells and sensitized them to CD95-mediated apoptosis. This study therefore implicates the LUCA-15 gene locus in the control of apoptosis.

Original language	English
Pages (from-to)	3774-3781
Number of pages	8
Journal	Oncogene
Volume	19
Issue number	33
DOIs	https://doi.org/10.1038/sj.onc.1203720
Publication status	Published - 28 Jul 2000

Keywords

Antisense
Apoptosis: chromosome 3
CD95
Jurkat
LUCA-15

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/sj.onc.1203720

Cite this

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title = "LUCA-I5-encoded sequence variants regulate CD95-mediated apoptosis",

abstract = "Using an expression cloning system to discover novel genes involved in apoptosis, we identified a 326 bp bone marrow cDNA fragment (termed Je2) that suppresses, upon transfection, CD95-mediated apoptosis in Jurkat T cells. Sequence homology revealed that Je2 maps to 3p21.3, to an intronic region of the candidate TSG LUCA-15 locus. It represents, in fact, an antisense transcript to the 3'-UTR of two novel splice variants of this gene. Overexpression of sequence representing one of these splice variants (a 2.6 kb cDNA termed Clone 26), inhibited proliferation of Jurkat cells and sensitized them to CD95-mediated apoptosis. This study therefore implicates the LUCA-15 gene locus in the control of apoptosis.",

keywords = "Antisense, Apoptosis: chromosome 3, CD95, Jurkat, LUCA-15",

author = "Sutherland, {Leslie C.} and Edwards, {Sandra E.} and Cable, {Hazel C.} and Poirier, {Guy G.} and Miller, {Barbara A.} and Cooper, {Colin S.} and Williams, {Gwyn T.}",

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T1 - LUCA-I5-encoded sequence variants regulate CD95-mediated apoptosis

AU - Sutherland, Leslie C.

AU - Edwards, Sandra E.

AU - Cable, Hazel C.

AU - Poirier, Guy G.

AU - Miller, Barbara A.

AU - Cooper, Colin S.

AU - Williams, Gwyn T.

PY - 2000/7/28

Y1 - 2000/7/28

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AB - Using an expression cloning system to discover novel genes involved in apoptosis, we identified a 326 bp bone marrow cDNA fragment (termed Je2) that suppresses, upon transfection, CD95-mediated apoptosis in Jurkat T cells. Sequence homology revealed that Je2 maps to 3p21.3, to an intronic region of the candidate TSG LUCA-15 locus. It represents, in fact, an antisense transcript to the 3'-UTR of two novel splice variants of this gene. Overexpression of sequence representing one of these splice variants (a 2.6 kb cDNA termed Clone 26), inhibited proliferation of Jurkat cells and sensitized them to CD95-mediated apoptosis. This study therefore implicates the LUCA-15 gene locus in the control of apoptosis.

KW - Antisense

KW - Apoptosis: chromosome 3

KW - CD95

KW - Jurkat

KW - LUCA-15

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LUCA-I5-encoded sequence variants regulate CD95-mediated apoptosis

Abstract

Keywords

UN SDGs

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