Matrix metalloproteinase distribution during early corneal wound healing

B. Mulholland, S. J. Tuft, P. T. Khaw

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: To compare matrix metalloproteinase (MMP) localisation in anterior keratectomy (AK) and lamellar keratectomy (LK) wounds.

Methods: Wounds were produced in one eye of 24 rabbits. The AK wounds were made to approximately 120 mm in depth and then allowed to re-epithelialise. The LK wounds were of similar depth, but the anterior stroma and epithelium were replaced after a second deeper keratectomy had been performed. Immunohistochemistry was used to localise the MMP-1, -2, -3, and -9 at intervals from 4 h to 14 days following surgery. The contralateral eyes acted as controls.

Results: After an AK wound MMP-1 was present at the leading edge of migrating epithelium after 18 h, while MMP-2 and -9 were localised behind the advancing epithelial edge. The presence of these enzymes rapidly fell to low levels after epithelial closure. There was only faint MMP-3 localisation between days 3 and 7. After an LK wound, MMP-1, -3, and -9 were not detected in the stromal interface, but MMP-2 was present at all time points.

Conclusions: This study suggests that after an AK wound, MMP-1 is a key mediator of epithelial migration, while MMP-2 and -9, and to a lesser extent MMP-3, may participate in the remodelling of corneal stroma and the reformation of epithelial basement membrane. In contrast, an LK wound results in a much lower stimulus for MMP activation. The action of MMP-2 in stromal repair is thus partly independent of epithelial injury.

Original languageEnglish
Pages (from-to)584-588
Number of pages5
JournalEye
Volume19
Issue number5
Early online date27 Aug 2004
DOIs
Publication statusPublished - 1 May 2005

Keywords

  • cornea
  • corneal epithelium
  • metalloproteinases
  • wound healing
  • IN-SITU KERATOMILEUSIS
  • PHOTOREFRACTIVE KERATECTOMY
  • GELATINASE-B
  • GENE-EXPRESSION
  • IV COLLAGENASE
  • LASER
  • IMMUNOLOCALIZATION
  • TIMP-1
  • REPAIR
  • CHONDROCYTES

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