TY - JOUR
T1 - Mechanism of met oncogene activation
AU - Park, Morag
AU - Dean, Michael
AU - Cooper, Colin S.
AU - Schmidt, Martin
AU - O'Brien, Stephen J.
AU - Blair, Donald G.
AU - Vande Woude, George F.
N1 - Funding Information:
We are grateful to M. Barbacid and S. Hughes for helpful comments and to Karen Cannon and Bobbie Jones for typing the manuscript. This research was in part supported by the National Cancer Institute, DHHS, under contract NOI-CO-23909, with Litton Bionetics, inc. Michael Dean is a Leukemia Society of America postdoctoral fellow. Martin Schmidt is a Deutsche Forschungs Gemeinschaft postdoctoral fellow (SCHM 633/1-l).
PY - 1986/6/20
Y1 - 1986/6/20
N2 - The met oncogene activated in vitro by treatment of a human osteogenic sarcoma (HOS) cell line with N-methyl-N′-nitronitrosoguanidine (MNNG) is related to the tyrosine kinase gene family. Probes from the met oncogene locus recognize two distinct transcripts of 9.0 kb and 10.0 kb which are independently expressed in a cell-type-specific fashion. While the met proto-oncogene locus expresses the 9.0 kb RNA and maps to human chromosome 7q21-31, the locus expressing the 10.0 kb RNA, (tpr; translocated promoter region) maps to human chromosome 1. Both MNNG-HOS cells and met NIH 3T3 transformants express a novel 5.0 kb RNA which represents a hybrid transcript with 5′ sequences derived from tpr and 3′ sequences from the met proto-oncogene. Treating HOS cells in vitro with MNNG, a known clastogenic carcinogen, resulted in fusion of two chromosomally disparate loci, met and tpr, generating the active met oncogene.
AB - The met oncogene activated in vitro by treatment of a human osteogenic sarcoma (HOS) cell line with N-methyl-N′-nitronitrosoguanidine (MNNG) is related to the tyrosine kinase gene family. Probes from the met oncogene locus recognize two distinct transcripts of 9.0 kb and 10.0 kb which are independently expressed in a cell-type-specific fashion. While the met proto-oncogene locus expresses the 9.0 kb RNA and maps to human chromosome 7q21-31, the locus expressing the 10.0 kb RNA, (tpr; translocated promoter region) maps to human chromosome 1. Both MNNG-HOS cells and met NIH 3T3 transformants express a novel 5.0 kb RNA which represents a hybrid transcript with 5′ sequences derived from tpr and 3′ sequences from the met proto-oncogene. Treating HOS cells in vitro with MNNG, a known clastogenic carcinogen, resulted in fusion of two chromosomally disparate loci, met and tpr, generating the active met oncogene.
UR - https://www.scopus.com/pages/publications/0022472582
U2 - 10.1016/0092-8674(86)90564-7
DO - 10.1016/0092-8674(86)90564-7
M3 - Article
C2 - 2423252
AN - SCOPUS:0022472582
SN - 0092-8674
VL - 45
SP - 895
EP - 904
JO - Cell
JF - Cell
IS - 6
ER -