Mechanisms of iron- and O2-sensing by the [4Fe-4S] cluster of the global iron regulator RirA

Maria Teresa Pellicer Martinez, Jason Crack, Melissa Stewart, Justin Bradley, Dimitri A. Svistunenko, Andrew Johnston, Myles Cheesman, Jonathan Todd, Nick Le Brun

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Abstract

RirA is a global regulator of iron homeostasis in Rhizobium and related α-proteobacteria. In its [4Fe-4S] cluster-bound form it represses iron uptake by binding to IRO Box sequences upstream of RirA-regulated genes. Under low iron and/or aerobic conditions, [4Fe-4S] RirA undergoes cluster conversion/degradation to apo-RirA, which can no longer bind IRO Box sequences. Here, we apply time-resolved mass spectrometry and electron paramagnetic resonance spectroscopy to determine how the RirA cluster senses iron and O2. The data indicate that the key iron-sensing step is the O2-independent, reversible dissociation of Fe2+ from [4Fe-4S]2+ to form [3Fe-4S]0. The dissociation constant for this process was determined as Kd = ~3 µM, which is consistent with the sensing of ‘free’ iron in the cytoplasm. O2-sensing occurs through enhanced cluster degradation under aerobic conditions, via O2-mediated oxidation of the [3Fe-4S]0 intermediate to form [3Fe-4S]1+. This work provides a detailed mechanistic/functional view of an iron-responsive regulator.

Original languageEnglish
Article numbere47804
JournaleLife
Volume8
DOIs
Publication statusPublished - 17 Sep 2019

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