Methodology for the at-home collection of urine samples for prostate cancer detection

Martyn Webb; Kate Manley; Mireia Olivan; Ingrid Guldvik; Malgorzata Palczynska; Rachel Hurst; Shea P Connell; Ian Mills; Daniel Brewer; Robert Mills; Colin Cooper; Jeremy Clark

doi:10.2144/btn-2019-0092

Methodology for the at-home collection of urine samples for prostate cancer detection

Martyn Webb
, Kate Manley
, Mireia Olivan
, Ingrid Guldvik
, Malgorzata Palczynska
, Rachel Hurst
, Shea P Connell
, Ian Mills
, Daniel Brewer
, Robert Mills
, Colin Cooper
, Jeremy Clark (Lead Author)

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

36 Downloads (Pure)

Abstract

Urine from prostate cancer patients contains gene transcripts which have been used for prostate cancer diagnosis and prognosis. Archetypally patient urine samples have been collected after a digital rectal examination (DRE) of the prostate which was thought necessary to boost the levels of prostatic secretions in the urine. We herein describe methodology that allows urine to be collected by patients at home and then posted to a laboratory for analysis. RNA yields and quality were comparable to post-DRE urine, and there was improved sensitivity for the detection of TMPRSS2:ERG transcripts by RT-PCR. The At-Home Collection protocol has opened up the potential to perform large-scale prostate cancer studies without the inconvenience, cost, discomfort and expense of having to visit the clinic.

Original language	English
Pages (from-to)	65-71
Number of pages	7
Journal	Biotechniques
Volume	68
Issue number	2
Early online date	29 Nov 2019
DOIs	https://doi.org/10.2144/btn-2019-0092
Publication status	Published - 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.2144/btn-2019-0092Licence: CC BY-NC-ND

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Webb_Protocol
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Daniel Brewer
- D.Breweruea.acuk
- Norwich Medical School - Professor in Medical Bioinformatics and Genomics
- Metabolic Health - Member
- Cancer Studies - Member
Person: Research Group Member, Research Centre Member, Academic, Teaching and Research
Colin Cooper
- Colin.Cooperuea.acuk
- Norwich Medical School - Professor of Cancer Genetics
- Metabolic Health - Member
- Cancer Studies - Member
Person: Research Group Member, Research Centre Member, Academic, Teaching and Research

Cite this

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title = "Methodology for the at-home collection of urine samples for prostate cancer detection",

abstract = "Urine from prostate cancer patients contains gene transcripts which have been used for prostate cancer diagnosis and prognosis. Archetypally patient urine samples have been collected after a digital rectal examination (DRE) of the prostate which was thought necessary to boost the levels of prostatic secretions in the urine. We herein describe methodology that allows urine to be collected by patients at home and then posted to a laboratory for analysis. RNA yields and quality were comparable to post-DRE urine, and there was improved sensitivity for the detection of TMPRSS2:ERG transcripts by RT-PCR. The At-Home Collection protocol has opened up the potential to perform large-scale prostate cancer studies without the inconvenience, cost, discomfort and expense of having to visit the clinic.",

author = "Martyn Webb and Kate Manley and Mireia Olivan and Ingrid Guldvik and Malgorzata Palczynska and Rachel Hurst and Connell, \{Shea P\} and Ian Mills and Daniel Brewer and Robert Mills and Colin Cooper and Jeremy Clark",

year = "2019",

doi = "10.2144/btn-2019-0092",

language = "English",

volume = "68",

pages = "65--71",

journal = "Biotechniques",

issn = "0736-6205",

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T1 - Methodology for the at-home collection of urine samples for prostate cancer detection

AU - Webb, Martyn

AU - Manley, Kate

AU - Olivan, Mireia

AU - Guldvik, Ingrid

AU - Palczynska, Malgorzata

AU - Hurst, Rachel

AU - Connell, Shea P

AU - Mills, Ian

AU - Brewer, Daniel

AU - Mills, Robert

AU - Cooper, Colin

AU - Clark, Jeremy

PY - 2019

Y1 - 2019

N2 - Urine from prostate cancer patients contains gene transcripts which have been used for prostate cancer diagnosis and prognosis. Archetypally patient urine samples have been collected after a digital rectal examination (DRE) of the prostate which was thought necessary to boost the levels of prostatic secretions in the urine. We herein describe methodology that allows urine to be collected by patients at home and then posted to a laboratory for analysis. RNA yields and quality were comparable to post-DRE urine, and there was improved sensitivity for the detection of TMPRSS2:ERG transcripts by RT-PCR. The At-Home Collection protocol has opened up the potential to perform large-scale prostate cancer studies without the inconvenience, cost, discomfort and expense of having to visit the clinic.

AB - Urine from prostate cancer patients contains gene transcripts which have been used for prostate cancer diagnosis and prognosis. Archetypally patient urine samples have been collected after a digital rectal examination (DRE) of the prostate which was thought necessary to boost the levels of prostatic secretions in the urine. We herein describe methodology that allows urine to be collected by patients at home and then posted to a laboratory for analysis. RNA yields and quality were comparable to post-DRE urine, and there was improved sensitivity for the detection of TMPRSS2:ERG transcripts by RT-PCR. The At-Home Collection protocol has opened up the potential to perform large-scale prostate cancer studies without the inconvenience, cost, discomfort and expense of having to visit the clinic.

U2 - 10.2144/btn-2019-0092

DO - 10.2144/btn-2019-0092

M3 - Article

SN - 0736-6205

VL - 68

SP - 65

EP - 71

JO - Biotechniques

JF - Biotechniques

IS - 2

ER -

Methodology for the at-home collection of urine samples for prostate cancer detection

Abstract

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Daniel Brewer

Colin Cooper

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