MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development

Juliana Giusti, Danillo Pinhal, Simon Moxon, Camilla Lovaglio Campos, Andrea Munsterberg, Cesar Martins

Research output: Contribution to journalArticle

13 Citations (Scopus)
14 Downloads (Pure)

Abstract

Hox gene clusters encode a family of transcription factors that govern anterior posterior axis patterning during embryogenesis in all bilaterian animals. The time and place ofHox gene expression are largely determined by the relative position of each gene within its cluster. Furthermore, Hox genes were shown to have their expression fine-tuned by regulatory microRNAs (miRNAs). However, the mechanisms of miRNA-mediated regulation of these transcription factors during fish early development remain largely unknown. Here we have profiled three highly expressed miR-10 family members of Nile tilapia at early embryonic development, determined their genomic organization as well as performed functional experiments for validation of target genes. Quantitative analysis during developmental stages showed miR-10 family expression negatively correlates with the expression of HoxA3a, HoxB3a and HoxD10a genes, as expected for bona fide miRNA–mRNA interactions. Moreover, luciferase assays demonstrated that HoxB3a and HoxD10a are targeted by miR-10b-5p. Overall, our data indicate that the miR-10 family directly regulates members of the Hox gene family during Nile tilapia embryogenesis.
Original languageEnglish
Pages (from-to)12-18
Number of pages7
JournalMechanisms of Development
Volume140
Early online date12 Mar 2016
DOIs
Publication statusPublished - May 2016

Keywords

  • Oreochromis niloticus
  • embryos
  • miRNA
  • HoxA3a
  • HoxB3a
  • HoxD10a

Cite this