MinION nanopore sequencing identifies the position and structure of a bacterial antibiotic resistance island

Philip Ashton, Satheesh Nair, Tim Dallman, Salvatore Rubino, Wolfgang Rabsch, Solomon Mwaigwisya, John Wain, Justin O'Grady

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328 Citations (Scopus)
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Short-read, high-throughput sequencing technology cannot identify the chromosomal position of repetitive insertion sequences that typically flank horizontally acquired genes such as bacterial virulence genes and antibiotic resistance genes. The MinION nanopore sequencer can produce long sequencing reads on a device similar in size to a USB memory stick. Here we apply a MinION sequencer to resolve the structure and chromosomal insertion site of a composite antibiotic resistance island in Salmonella Typhi Haplotype 58. Nanopore sequencing data from a single 18-h run was used to create a scaffold for an assembly generated from short-read Illumina data. Our results demonstrate the potential of the MinION device in clinical laboratories to fully characterize the epidemic spread of bacterial pathogens.
Original languageEnglish
Pages (from-to)296–300
Number of pages5
JournalNature Biotechnology
Issue number3
Early online date8 Dec 2014
Publication statusPublished - Mar 2015

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