Modulated kinase activities, in cells undergoing tumour necrosis, factor-induced apoptotic cell death

M. L. Helms; A. A. A. Mohamed; D. J. Macewan

Modulated kinase activities, in cells undergoing tumour necrosis, factor-induced apoptotic cell death

M. L. Helms, A. A. A. Mohamed, D. J. Macewan

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Tumour necrosis, factor-alpha (TNF) has, a variety of cellular effects including apoptotic, and necrotic cytotoxicity. TNF activates a range of kinases, but their role in cytotoxic mechanisms is unclear. HeLa cells expressing elevated type II 75 kDa TNF receptor (TNFR2) protein, analysed by flow cytometry and Western analysis, showed altered c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK; but not MAPK) protein content and activation. There was greater JNK activation, but reduced p38MAPK activation in dying cells compared to those still to enter TNF-induced apoptosis. Moreover, cells displaying more rapid apoptosis possess higher levels of type I 55 kDa TNFR1 receptor isoform, but less TNFR2. These findings reveal differential kinase activation in TNF-induced apoptotic death. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	68-74
Number of pages	7
Journal	FEBS Letters
Volume	505
Issue number	1
Publication status	Published - 2001

Keywords

CYTOSOLIC PHOSPHOLIPASE
tumor
N-TERMINAL KINASE
CYTOCHROME-C
signal transduction
FACTOR-ALPHA
PHORBOL ESTER
kinase
TNF RECEPTOR
ACTIVATED PROTEIN-KINASE
SIGNAL-TRANSDUCTION
cytokine
receptor
JNK ACTIVATION
subtype
A(2)
HELA-CELLS

Cite this

@article{576a2971c5604f60b1bc73c54c538591,

title = "Modulated kinase activities, in cells undergoing tumour necrosis, factor-induced apoptotic cell death",

abstract = "Tumour necrosis, factor-alpha (TNF) has, a variety of cellular effects including apoptotic, and necrotic cytotoxicity. TNF activates a range of kinases, but their role in cytotoxic mechanisms is unclear. HeLa cells expressing elevated type II 75 kDa TNF receptor (TNFR2) protein, analysed by flow cytometry and Western analysis, showed altered c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK; but not MAPK) protein content and activation. There was greater JNK activation, but reduced p38MAPK activation in dying cells compared to those still to enter TNF-induced apoptosis. Moreover, cells displaying more rapid apoptosis possess higher levels of type I 55 kDa TNFR1 receptor isoform, but less TNFR2. These findings reveal differential kinase activation in TNF-induced apoptotic death. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.",

keywords = "CYTOSOLIC PHOSPHOLIPASE, tumor, N-TERMINAL KINASE, CYTOCHROME-C, signal transduction, FACTOR-ALPHA, PHORBOL ESTER, kinase, TNF RECEPTOR, ACTIVATED PROTEIN-KINASE, SIGNAL-TRANSDUCTION, cytokine, receptor, JNK ACTIVATION, subtype, A(2), HELA-CELLS",

author = "Helms, {M. L.} and Mohamed, {A. A. A.} and Macewan, {D. J.}",

year = "2001",

language = "English",

volume = "505",

pages = "68--74",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Modulated kinase activities, in cells undergoing tumour necrosis, factor-induced apoptotic cell death

AU - Helms, M. L.

AU - Mohamed, A. A. A.

AU - Macewan, D. J.

PY - 2001

Y1 - 2001

N2 - Tumour necrosis, factor-alpha (TNF) has, a variety of cellular effects including apoptotic, and necrotic cytotoxicity. TNF activates a range of kinases, but their role in cytotoxic mechanisms is unclear. HeLa cells expressing elevated type II 75 kDa TNF receptor (TNFR2) protein, analysed by flow cytometry and Western analysis, showed altered c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK; but not MAPK) protein content and activation. There was greater JNK activation, but reduced p38MAPK activation in dying cells compared to those still to enter TNF-induced apoptosis. Moreover, cells displaying more rapid apoptosis possess higher levels of type I 55 kDa TNFR1 receptor isoform, but less TNFR2. These findings reveal differential kinase activation in TNF-induced apoptotic death. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

AB - Tumour necrosis, factor-alpha (TNF) has, a variety of cellular effects including apoptotic, and necrotic cytotoxicity. TNF activates a range of kinases, but their role in cytotoxic mechanisms is unclear. HeLa cells expressing elevated type II 75 kDa TNF receptor (TNFR2) protein, analysed by flow cytometry and Western analysis, showed altered c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK; but not MAPK) protein content and activation. There was greater JNK activation, but reduced p38MAPK activation in dying cells compared to those still to enter TNF-induced apoptosis. Moreover, cells displaying more rapid apoptosis possess higher levels of type I 55 kDa TNFR1 receptor isoform, but less TNFR2. These findings reveal differential kinase activation in TNF-induced apoptotic death. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

KW - CYTOSOLIC PHOSPHOLIPASE

KW - tumor

KW - N-TERMINAL KINASE

KW - CYTOCHROME-C

KW - signal transduction

KW - FACTOR-ALPHA

KW - PHORBOL ESTER

KW - kinase

KW - TNF RECEPTOR

KW - ACTIVATED PROTEIN-KINASE

KW - SIGNAL-TRANSDUCTION

KW - cytokine

KW - receptor

KW - JNK ACTIVATION

KW - subtype

KW - A(2)

KW - HELA-CELLS

M3 - Article

VL - 505

SP - 68

EP - 74

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -