Molecular epidemiology of fluoroquinolone-resistant ST131 Escherichia coli producing CTX-M extended-spectrum -lactamases in nursing homes in Belfast, UK

Hiran Dhanji, Michel Doumith, Paul J Rooney, Maureen C O'Leary, Anne C Loughrey, Russell Hope, Neil Woodford, David M Livermore

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

Objectives Between January 2004 and May 2006 Escherichia coli producing extended-spectrum ß-lactamases (ESBLs) were isolated from the faeces of 118/294 residents from 16 nursing homes in Belfast. Of these, 58 isolates belonged to UK strain A, a variant of the international ST131 clone. Here we investigated the remaining 60 ESBL producers. Methods MICs were determined and interpreted using BSAC methodology. Isolates were characterized by phylogenetic typing, real-time PCR and PFGE. Plasmids were rep typed by PCR and their similarity to IncI1 reference plasmid pEK204 was investigated by restriction fragment length polymorphism analysis. The molecular environments surrounding blaCTX-M were determined by DNA sequencing and PCR. Results Fifty-nine of 60 isolates belonged to the B2, ST131 lineage; of these 28 belonged to the previously defined UK strain C, while the other 31 were clustered into five groups by PFGE. Forty-nine isolates harboured blaCTX-M-3 on plasmids of five different rep types (I1, FIA, FIA-FIB, N and Y) and 11 harboured blaCTX-M-15 on F-type plasmids (FIA and FIA-FIB). All CTX-M-3 ESBL producers and three with CTX-M-15 ESBL had an intact copy of ISEcp1 immediately upstream of blaCTX-M; the remaining eight with CTX-M-15 ESBL had a truncated ISEcp1. Conclusions Gut colonization among nursing home residents in Belfast with ciprofloxacin-resistant E. coli producing ESBLs almost entirely involves clonal spread of ST131 variants, with similar genetic environments for blaCTX-M-3 or blaCTX-M-15 as in pEK204 and pEK499. Such diversity indicates dissemination of both plasmids and ESBL genes among a single commonly multiresistant clone.
Original languageEnglish
Pages (from-to)297-303
Number of pages7
JournalJournal of Antimicrobial Chemotherapy
Volume66
Issue number2
DOIs
Publication statusPublished - Feb 2011

Keywords

  • Cephalosporin Resistance
  • Ciprofloxacin
  • Escherichia coli
  • Escherichia coli Infections
  • Feces
  • Fluoroquinolones
  • Great Britain
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Epidemiology
  • Molecular Typing
  • Nursing Homes
  • Phylogeny
  • Plasmids
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Sequence Analysis, DNA
  • beta-Lactamases

Cite this