mRNA expression profiling reveals conserved and non-conserved miR-140 targets

Francisco Esteban Nicolas, Helio Pais, Frank Schwach, Morten Lindow, Sakari Kauppinen, Vincent Moulton, Tamas Dalmay

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

microRNAs are non-coding RNAs that regulate gene expression. A significant proportion of microRNAs is perfectly conserved across the vertebrate clade, including miR-140, which is specifically expressed in cartilage. Although it has been computationally predicted that a large majority of microRNA targets are conserved, experimental evidence for this hypothesis remains scarce. In this work we use mRNA expression profiles obtained after manipulation of miR-140 activity levels in human and chicken primary chondrocytes to explore the extent of miR-140 target conservation. Our data suggest that miR-140 has a large number of targets conserved between human and chicken and we validate one of these, BMP2. However, we also found a significant number of non-conserved targets in the two species. In addition, we found that a commercially available scrambled siRNA, which is regularly used as a negative control, regulate the accumulation of many genes.
Original languageEnglish
Pages (from-to)607-615
Number of pages9
JournalRNA Biology
Volume8
Issue number4
DOIs
Publication statusPublished - 1 Jul 2011

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