Multicenter blinded analysis of RT-PCR detection methods for paramyxoviruses in relation to Paget's disease of bone

Stuart H Ralston, Muhammad A Afzal, Miep H Helfrich, William D Fraser, James A Gallagher, Andrew Mee, Bert Rima

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Abstract

Conflicting results have been reported on the detection of paramyxovirus transcripts in Paget's disease, and a possible explanation is differences in the sensitivity of RT-PCR methods for detecting virus. In a blinded study, we found no evidence to suggest that laboratories that failed to detect viral transcripts had less sensitive RT-PCR assays, and we did not detect measles or distemper transcripts in Paget's samples using the most sensitive assays evaluated.
Original languageEnglish
Pages (from-to)569-577
Number of pages9
JournalJournal of Bone and Mineral Research
Volume22
Issue number4
DOIs
Publication statusPublished - Apr 2007

Keywords

  • Base Sequence
  • Bone and Bones
  • DNA Primers
  • Distemper Virus, Canine
  • Humans
  • Laboratories
  • Leukocytes, Mononuclear
  • Measles virus
  • Osteitis Deformans
  • Paramyxoviridae Infections
  • Paramyxovirinae
  • RNA, Viral
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity

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