Multicenter blinded analysis of RT-PCR detection methods for paramyxoviruses in relation to Paget's disease of bone

Stuart H Ralston; Muhammad A Afzal; Miep H Helfrich; William D Fraser; James A Gallagher; Andrew Mee; Bert Rima

doi:10.1359/jbmr.070103

Multicenter blinded analysis of RT-PCR detection methods for paramyxoviruses in relation to Paget's disease of bone

Stuart H Ralston, Muhammad A Afzal, Miep H Helfrich, William D Fraser, James A Gallagher, Andrew Mee, Bert Rima

Research output: Contribution to journal › Article › peer-review

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Abstract

Conflicting results have been reported on the detection of paramyxovirus transcripts in Paget's disease, and a possible explanation is differences in the sensitivity of RT-PCR methods for detecting virus. In a blinded study, we found no evidence to suggest that laboratories that failed to detect viral transcripts had less sensitive RT-PCR assays, and we did not detect measles or distemper transcripts in Paget's samples using the most sensitive assays evaluated.

Original language	English
Pages (from-to)	569-577
Number of pages	9
Journal	Journal of Bone and Mineral Research
Volume	22
Issue number	4
DOIs	https://doi.org/10.1359/jbmr.070103
Publication status	Published - Apr 2007

Keywords

Base Sequence
Bone and Bones
DNA Primers
Distemper Virus, Canine
Humans
Laboratories
Leukocytes, Mononuclear
Measles virus
Osteitis Deformans
Paramyxoviridae Infections
Paramyxovirinae
RNA, Viral
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1359/jbmr.070103

Cite this

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abstract = "Conflicting results have been reported on the detection of paramyxovirus transcripts in Paget's disease, and a possible explanation is differences in the sensitivity of RT-PCR methods for detecting virus. In a blinded study, we found no evidence to suggest that laboratories that failed to detect viral transcripts had less sensitive RT-PCR assays, and we did not detect measles or distemper transcripts in Paget's samples using the most sensitive assays evaluated.",

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AU - Ralston, Stuart H

AU - Afzal, Muhammad A

AU - Helfrich, Miep H

AU - Fraser, William D

AU - Gallagher, James A

AU - Mee, Andrew

AU - Rima, Bert

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KW - DNA Primers

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KW - Laboratories

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KW - Osteitis Deformans

KW - Paramyxoviridae Infections

KW - Paramyxovirinae

KW - RNA, Viral

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