Multicenter blinded analysis of RT-PCR detection methods for paramyxoviruses in relation to Paget's disease of bone

Stuart H Ralston; Muhammad A Afzal; Miep H Helfrich; William D Fraser; James A Gallagher; Andrew Mee; Bert Rima

doi:10.1359/jbmr.070103

Multicenter blinded analysis of RT-PCR detection methods for paramyxoviruses in relation to Paget's disease of bone

Stuart H Ralston
, Muhammad A Afzal
, Miep H Helfrich
, William D Fraser
, James A Gallagher
, Andrew Mee
, Bert Rima

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

Conflicting results have been reported on the detection of paramyxovirus transcripts in Paget's disease, and a possible explanation is differences in the sensitivity of RT-PCR methods for detecting virus. In a blinded study, we found no evidence to suggest that laboratories that failed to detect viral transcripts had less sensitive RT-PCR assays, and we did not detect measles or distemper transcripts in Paget's samples using the most sensitive assays evaluated.

Original language	English
Pages (from-to)	569-577
Number of pages	9
Journal	Journal of Bone and Mineral Research
Volume	22
Issue number	4
DOIs	https://doi.org/10.1359/jbmr.070103
Publication status	Published - Apr 2007

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Base Sequence
Bone and Bones
DNA Primers
Distemper Virus, Canine
Humans
Laboratories
Leukocytes, Mononuclear
Measles virus
Osteitis Deformans
Paramyxoviridae Infections
Paramyxovirinae
RNA, Viral
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity

Access to Document

10.1359/jbmr.070103

Cite this

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title = "Multicenter blinded analysis of RT-PCR detection methods for paramyxoviruses in relation to Paget's disease of bone",

abstract = "Conflicting results have been reported on the detection of paramyxovirus transcripts in Paget's disease, and a possible explanation is differences in the sensitivity of RT-PCR methods for detecting virus. In a blinded study, we found no evidence to suggest that laboratories that failed to detect viral transcripts had less sensitive RT-PCR assays, and we did not detect measles or distemper transcripts in Paget's samples using the most sensitive assays evaluated.",

keywords = "Base Sequence, Bone and Bones, DNA Primers, Distemper Virus, Canine, Humans, Laboratories, Leukocytes, Mononuclear, Measles virus, Osteitis Deformans, Paramyxoviridae Infections, Paramyxovirinae, RNA, Viral, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity",

author = "Ralston, \{Stuart H\} and Afzal, \{Muhammad A\} and Helfrich, \{Miep H\} and Fraser, \{William D\} and Gallagher, \{James A\} and Andrew Mee and Bert Rima",

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AU - Ralston, Stuart H

AU - Afzal, Muhammad A

AU - Helfrich, Miep H

AU - Fraser, William D

AU - Gallagher, James A

AU - Mee, Andrew

AU - Rima, Bert

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AB - Conflicting results have been reported on the detection of paramyxovirus transcripts in Paget's disease, and a possible explanation is differences in the sensitivity of RT-PCR methods for detecting virus. In a blinded study, we found no evidence to suggest that laboratories that failed to detect viral transcripts had less sensitive RT-PCR assays, and we did not detect measles or distemper transcripts in Paget's samples using the most sensitive assays evaluated.

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KW - DNA Primers

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KW - Humans

KW - Laboratories

KW - Leukocytes, Mononuclear

KW - Measles virus

KW - Osteitis Deformans

KW - Paramyxoviridae Infections

KW - Paramyxovirinae

KW - RNA, Viral

KW - Reverse Transcriptase Polymerase Chain Reaction

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