Multifrequency cw-EPR investigation of the catalytic molybdenum cofactor of polysulfide reductase from Wolinella succinogenes

Thomas Prisner (Lead Author), Sevdalina Lyubenova, Yener Atabay, Fraser MacMillan, Achim Kröger, Oliver Klimmek

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Electron paramagnetic resonance (EPR) spectra of the molybdenum centre in polysulfide reductase (Psr) from Wolinella succinogenes with unusually high G-tensor values have been observed for the first time. Three different Mo states have been generated (by the addition of the substrate polysulfide and different redox agents) and analysed by their G- and hyperfine tensors using multifrequency (S-, X- and Q-band) cw-EPR spectroscopy. The unusually high G-tensor values are attributed to a large number of sulfur ligands. Four sulfur ligands are assumed to arise from two pterin cofactors; one additional sulfur ligand was identified from mutagenesis studies to be a cysteine residue of the protein backbone. One further sulfur ligand is proposed for two of the Mo states, based on the experimentally observed shift of the g value. This sixth sulfur ligand is postulated to belong to the polysulfide substrate consumed within the catalytic reaction cycle of the enzyme. The influence of the co-protein sulfur transferase on the Mo G-tensor supports this assignment.
Original languageEnglish
Pages (from-to)419-426
Number of pages8
JournalJBIC Journal of Biological Inorganic Chemistry
Issue number4
Publication statusPublished - 1 Apr 2003

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