Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries

Seon Young Choi; Yoon-Seong Jeon; Je Hee Lee; Boram Choi; Sun Hwa Moon; Lorenz von Seidlein; John D. Clemens; Gordon Dougan; John Wain; Jun Yu; Je Chul Lee; Sung Yong Seol; Bok Kwon Lee; Jae-Hoon Song; Manki Song; Cecil Czerkinsky; Jongsik Chun; Dong Wook Kim

doi:10.1099/jmm.0.47322-0

Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries

Seon Young Choi, Yoon-Seong Jeon, Je Hee Lee, Boram Choi, Sun Hwa Moon, Lorenz von Seidlein, John D. Clemens, Gordon Dougan, John Wain, Jun Yu, Je Chul Lee, Sung Yong Seol, Bok Kwon Lee, Jae-Hoon Song, Manki Song, Cecil Czerkinsky, Jongsik Chun, Dong Wook Kim

Norwich Medical School

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

The multilocus sequence typing scheme used previously for phylogenetic analysis of Escherichia coli was applied to 107 clinical isolates of Shigella flexneri. DNA sequencing of 3423 bp throughout seven housekeeping genes identified eight new allele types and ten new sequence types among the isolates. S. flexneri serotypes 1–5, X and Y were clustered together in a group containing many allelic variants while serotype 6 formed a distinct group, as previously established. EIEC, enteroinvasive E. coliMLST, multilocus sequence typingST, sequence type The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences of the new allele types identified in this study are EF364101–EF364108 (icd123, EF364101; adk116, EF364102; fumC149, EF364103; fumC145, EF364104; purA98, EF364105; purA96, EF364106; purA97, EF364107; purA95, EF364108). A full list of clinical S. flexneri isolates subjected to MLST analysis in this study and a phylogenetic tree generated by the neighbour-joining method for S. flexneri are available as supplementary material with the online version of this paper.

Original language	English
Pages (from-to)	1460-1466
Number of pages	7
Journal	Journal of Medical Microbiology
Volume	56
Issue number	11
DOIs	https://doi.org/10.1099/jmm.0.47322-0
Publication status	Published - Nov 2007

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Choi, S. Y., Jeon, Y-S., Lee, J. H., Choi, B., Moon, S. H., von Seidlein, L., Clemens, J. D., Dougan, G., Wain, J., Yu, J., Lee, J. C., Seol, S. Y., Lee, B. K., Song, J-H., Song, M., Czerkinsky, C., Chun, J., & Kim, D. W. (2007). Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries. Journal of Medical Microbiology, 56(11), 1460-1466. https://doi.org/10.1099/jmm.0.47322-0

@article{251a0be49ce24634a44f1e8a704e402f,

title = "Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries",

abstract = "The multilocus sequence typing scheme used previously for phylogenetic analysis of Escherichia coli was applied to 107 clinical isolates of Shigella flexneri. DNA sequencing of 3423 bp throughout seven housekeeping genes identified eight new allele types and ten new sequence types among the isolates. S. flexneri serotypes 1–5, X and Y were clustered together in a group containing many allelic variants while serotype 6 formed a distinct group, as previously established. EIEC, enteroinvasive E. coliMLST, multilocus sequence typingST, sequence type The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences of the new allele types identified in this study are EF364101–EF364108 (icd123, EF364101; adk116, EF364102; fumC149, EF364103; fumC145, EF364104; purA98, EF364105; purA96, EF364106; purA97, EF364107; purA95, EF364108). A full list of clinical S. flexneri isolates subjected to MLST analysis in this study and a phylogenetic tree generated by the neighbour-joining method for S. flexneri are available as supplementary material with the online version of this paper.",

author = "Choi, {Seon Young} and Yoon-Seong Jeon and Lee, {Je Hee} and Boram Choi and Moon, {Sun Hwa} and {von Seidlein}, Lorenz and Clemens, {John D.} and Gordon Dougan and John Wain and Jun Yu and Lee, {Je Chul} and Seol, {Sung Yong} and Lee, {Bok Kwon} and Jae-Hoon Song and Manki Song and Cecil Czerkinsky and Jongsik Chun and Kim, {Dong Wook}",

year = "2007",

month = nov,

doi = "10.1099/jmm.0.47322-0",

language = "English",

volume = "56",

pages = "1460--1466",

journal = "Journal of Medical Microbiology",

issn = "0022-2615",

publisher = "Society for General Microbiology",

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Choi, SY, Jeon, Y-S, Lee, JH, Choi, B, Moon, SH, von Seidlein, L, Clemens, JD, Dougan, G, Wain, J, Yu, J, Lee, JC, Seol, SY, Lee, BK, Song, J-H, Song, M, Czerkinsky, C, Chun, J & Kim, DW 2007, 'Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries', Journal of Medical Microbiology, vol. 56, no. 11, pp. 1460-1466. https://doi.org/10.1099/jmm.0.47322-0

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T1 - Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries

AU - Choi, Seon Young

AU - Jeon, Yoon-Seong

AU - Lee, Je Hee

AU - Choi, Boram

AU - Moon, Sun Hwa

AU - von Seidlein, Lorenz

AU - Clemens, John D.

AU - Dougan, Gordon

AU - Wain, John

AU - Yu, Jun

AU - Lee, Je Chul

AU - Seol, Sung Yong

AU - Lee, Bok Kwon

AU - Song, Jae-Hoon

AU - Song, Manki

AU - Czerkinsky, Cecil

AU - Chun, Jongsik

AU - Kim, Dong Wook

PY - 2007/11

Y1 - 2007/11

N2 - The multilocus sequence typing scheme used previously for phylogenetic analysis of Escherichia coli was applied to 107 clinical isolates of Shigella flexneri. DNA sequencing of 3423 bp throughout seven housekeeping genes identified eight new allele types and ten new sequence types among the isolates. S. flexneri serotypes 1–5, X and Y were clustered together in a group containing many allelic variants while serotype 6 formed a distinct group, as previously established. EIEC, enteroinvasive E. coliMLST, multilocus sequence typingST, sequence type The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences of the new allele types identified in this study are EF364101–EF364108 (icd123, EF364101; adk116, EF364102; fumC149, EF364103; fumC145, EF364104; purA98, EF364105; purA96, EF364106; purA97, EF364107; purA95, EF364108). A full list of clinical S. flexneri isolates subjected to MLST analysis in this study and a phylogenetic tree generated by the neighbour-joining method for S. flexneri are available as supplementary material with the online version of this paper.

AB - The multilocus sequence typing scheme used previously for phylogenetic analysis of Escherichia coli was applied to 107 clinical isolates of Shigella flexneri. DNA sequencing of 3423 bp throughout seven housekeeping genes identified eight new allele types and ten new sequence types among the isolates. S. flexneri serotypes 1–5, X and Y were clustered together in a group containing many allelic variants while serotype 6 formed a distinct group, as previously established. EIEC, enteroinvasive E. coliMLST, multilocus sequence typingST, sequence type The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences of the new allele types identified in this study are EF364101–EF364108 (icd123, EF364101; adk116, EF364102; fumC149, EF364103; fumC145, EF364104; purA98, EF364105; purA96, EF364106; purA97, EF364107; purA95, EF364108). A full list of clinical S. flexneri isolates subjected to MLST analysis in this study and a phylogenetic tree generated by the neighbour-joining method for S. flexneri are available as supplementary material with the online version of this paper.

U2 - 10.1099/jmm.0.47322-0

DO - 10.1099/jmm.0.47322-0

M3 - Article

VL - 56

SP - 1460

EP - 1466

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - 11

ER -