Multiple FLC haplotypes defined by independent cis-regulatory variation underpin life history diversity in Arabidopsis thaliana

Peijin Li; Daniele Filiault; Mathew S. Box; Envel Kerdaffrec; Cock van Oosterhout; Amity M. Wilczek; Johanna Schmitt; Mark McMullan; Joy Bergelson; Magnus Nordborg; Caroline Dean

doi:10.1101/gad.245993.114

Multiple FLC haplotypes defined by independent cis-regulatory variation underpin life history diversity in Arabidopsis thaliana

Peijin Li, Daniele Filiault, Mathew S. Box, Envel Kerdaffrec, Cock van Oosterhout, Amity M. Wilczek, Johanna Schmitt, Mark McMullan, Joy Bergelson, Magnus Nordborg, Caroline Dean

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Abstract

Relating molecular variation to phenotypic diversity is a central goal in evolutionary biology. In Arabidopsis thaliana, FLOWERING LOCUS C (FLC) is a major determinant of variation in vernalization—the acceleration of flowering by prolonged cold. Here, through analysis of 1307 A. thaliana accessions, we identify five predominant FLC haplotypes defined by noncoding sequence variation. Genetic and transgenic experiments show that they are functionally distinct, varying in FLC expression level and rate of epigenetic silencing. Allelic heterogeneity at this single locus accounts for a large proportion of natural variation in vernalization that contributes to adaptation of A. thaliana.

Original language	English
Pages (from-to)	1635-1640
Number of pages	6
Journal	Genes & Development
Volume	28
Issue number	15
Early online date	17 Jul 2014
DOIs	https://doi.org/10.1101/gad.245993.114
Publication status	Published - 1 Aug 2014

Keywords

allelic heterogeneity
FLOWERING LOCUS C
noncoding polymorphism
vernalization
adaptation

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10.1101/gad.245993.114

Li_etal_2014_GenesDev
© 2014 Li et al.; Published by Cold Spring Harbor Laboratory Press This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0.
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Cite this

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title = "Multiple FLC haplotypes defined by independent cis-regulatory variation underpin life history diversity in Arabidopsis thaliana",

abstract = "Relating molecular variation to phenotypic diversity is a central goal in evolutionary biology. In Arabidopsis thaliana, FLOWERING LOCUS C (FLC) is a major determinant of variation in vernalization—the acceleration of flowering by prolonged cold. Here, through analysis of 1307 A. thaliana accessions, we identify five predominant FLC haplotypes defined by noncoding sequence variation. Genetic and transgenic experiments show that they are functionally distinct, varying in FLC expression level and rate of epigenetic silencing. Allelic heterogeneity at this single locus accounts for a large proportion of natural variation in vernalization that contributes to adaptation of A. thaliana. ",

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AU - Li, Peijin

AU - Filiault, Daniele

AU - Box, Mathew S.

AU - Kerdaffrec, Envel

AU - van Oosterhout, Cock

AU - Wilczek, Amity M.

AU - Schmitt, Johanna

AU - McMullan, Mark

AU - Bergelson, Joy

AU - Nordborg, Magnus

AU - Dean, Caroline

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