Multiple recognition of RXLR effectors is associated with nonhost resistance of pepper against Phytophthora infestans

Hyun Ah Lee, Shin Young Kim, Sang Keun Oh, Seon In Yeom, Saet Byul Kim, Myung Shin Kim, Sophien Kamoun, Doil Choi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Summary: Nonhost resistance (NHR) is a plant immune response to resist most pathogens. The molecular basis of NHR is poorly understood, but recognition of pathogen effectors by immune receptors, a response known as effector-triggered immunity, has been proposed as a component of NHR. We performed transient expression of 54 Phytophthora infestansRXLR effectors in pepper (Capsicum annuum) accessions. We used optimized heterologous expression methods and analyzed the inheritance of effector-induced cell death in an F2 population derived from a cross between two pepper accessions. Pepper showed a localized cell death response upon inoculation with P. infestans, suggesting that recognition of effectors may contribute to NHR in this system. Pepper accessions recognized as many as 36 effectors. Among the effectors, PexRD8 and Avrblb2 induced cell death in a broad range of pepper accessions. Segregation of effector-induced cell death in an F2 population derived from a cross between two pepper accessions fit 15 : 1, 9 : 7 or 3 : 1 ratios, depending on the effector. Our genetic data suggest that a single or two independent/complementary dominant genes are involved in the recognition of RXLR effectors. Multiple loci recognizing a series of effectors may underpin NHR of pepper to P. infestans and confer resistance durability.

Original languageEnglish
Pages (from-to)926-938
Number of pages13
JournalNew Phytologist
Volume203
Issue number3
DOIs
Publication statusPublished - 2014

Keywords

  • Multiple interactions
  • Nonhost resistance
  • Pepper
  • Phytophthora infestans
  • RXLR effectors

Cite this