Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.

Neil Woodford; Matthew J. Ellington; Juliana Coelho; Jane F. Turton; M. Elaina Ward; Susan Brown; Sebastian G. B. Amyes; David M. Livermore

doi:10.1016/j.ijantimicag.2006.01.004

Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.

Neil Woodford, Matthew J. Ellington, Juliana Coelho, Jane F. Turton, M. Elaina Ward, Susan Brown, Sebastian G. B. Amyes, David M. Livermore

Research output: Contribution to journal › Article › peer-review

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Abstract

Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii ‘OXA’ clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the ‘SE clone’ had only an intrinsic blaOXA-51-like allele. Genes encoding OXA-58 were detected (with blaOXA-51-like) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.

Original language	English
Pages (from-to)	351-353
Number of pages	3
Journal	International Journal of Antimicrobial Agents
Volume	27
Issue number	4
DOIs	https://doi.org/10.1016/j.ijantimicag.2006.01.004
Publication status	Published - Apr 2006

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10.1016/j.ijantimicag.2006.01.004

Cite this

@article{231cecf932f243ba997e95a3107dcfb6,

title = "Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.",

abstract = "Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii {\textquoteleft}OXA{\textquoteright} clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the {\textquoteleft}SE clone{\textquoteright} had only an intrinsic blaOXA-51-like allele. Genes encoding OXA-58 were detected (with blaOXA-51-like) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.",

author = "Neil Woodford and Ellington, {Matthew J.} and Juliana Coelho and Turton, {Jane F.} and Ward, {M. Elaina} and Susan Brown and Amyes, {Sebastian G. B.} and Livermore, {David M.}",

year = "2006",

month = apr,

doi = "10.1016/j.ijantimicag.2006.01.004",

language = "English",

volume = "27",

pages = "351--353",

journal = "International Journal of Antimicrobial Agents",

issn = "0924-8579",

publisher = "Elsevier",

number = "4",

}

TY - JOUR

T1 - Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.

AU - Woodford, Neil

AU - Ellington, Matthew J.

AU - Coelho, Juliana

AU - Turton, Jane F.

AU - Ward, M. Elaina

AU - Brown, Susan

AU - Amyes, Sebastian G. B.

AU - Livermore, David M.

PY - 2006/4

Y1 - 2006/4

N2 - Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii ‘OXA’ clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the ‘SE clone’ had only an intrinsic blaOXA-51-like allele. Genes encoding OXA-58 were detected (with blaOXA-51-like) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.

AB - Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii ‘OXA’ clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the ‘SE clone’ had only an intrinsic blaOXA-51-like allele. Genes encoding OXA-58 were detected (with blaOXA-51-like) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.

U2 - 10.1016/j.ijantimicag.2006.01.004

DO - 10.1016/j.ijantimicag.2006.01.004

M3 - Article

VL - 27

SP - 351

EP - 353

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

IS - 4

ER -