Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.

Neil Woodford, Matthew J. Ellington, Juliana Coelho, Jane F. Turton, M. Elaina Ward, Susan Brown, Sebastian G. B. Amyes, David M. Livermore

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793 Citations (Scopus)

Abstract

Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii ‘OXA’ clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the ‘SE clone’ had only an intrinsic blaOXA-51-like allele. Genes encoding OXA-58 were detected (with blaOXA-51-like) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.
Original languageEnglish
Pages (from-to)351-353
Number of pages3
JournalInternational Journal of Antimicrobial Agents
Volume27
Issue number4
DOIs
Publication statusPublished - Apr 2006

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