Murine tissue inhibitor of metalloproteinases-4 (Timp-4): cDNA isolation and expression in adult mouse tissues

Kevin J. Leco, Suneel S. Apte, Gary T. Taniguchi, Susan P. Hawkes, Rama Khokha, Gilbert A. Schultz, Dylan R. Edwards

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We have isolated cDNA clones corresponding to a new member of the murine tissue inhibitor of metalloproteinase (TIMP) family, designated Timp-4. The nucleotide sequence predicts a protein of 22,609 Da that contains the characteristic 12 cysteine TIMP signature. TIMP-4 is more closely related to TIMP-2 and TIMP-3 than to TIMP-1 (48%, 45% and 38% identity, respectively). Analysis of Timp-4 mRNA expression in adult mouse tissues indicated a 1.2 kb transcript in brain, heart, ovary and skeletal muscle. This pattern of expression distinguishes Timp-4 from other Timps, suggesting that the TIMP-4 protein may be an important tissue-specific regulator of extracellular matrix remodelling.
Original languageEnglish
Pages (from-to)213-217
Number of pages5
JournalFEBS Letters
Issue number2-3
Publication statusPublished - 20 Jan 1997


  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Enzyme Inhibitors
  • Male
  • Metalloendopeptidases
  • Mice
  • Molecular Sequence Data
  • Proteins
  • RNA
  • Sequence Homology, Amino Acid
  • Tissue Inhibitor of Metalloproteinases

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